Manganese oxide-carbon quantum dots nano-composites for fluorescence/magnetic resonance (T1) dual mode bioimaging, long term cell tracking, and ROS scavenging

摘要

Multimodal long-term imaging probes with capability of extracting complementary information are highly important in biomedical engineering for disease diagnosis and monitoring of therapeutics distribution. However, most of the theranostics probes used are transient and have inherent problem of toxicity mostly related to generation of free radicals. In current study, a simple microwave assisted synthesis of multimodal imaging nanoprobe (T1 contrast in MR/fluorescence) is reported via doping carbon quantum dots into manganese oxide nanoparticles. The nanostructures were characterized by US-Vis spectroscopy, fluorescence spectroscopy, FTIR, Raman spectroscopy, TEM, XRD, AFM and XPS. The average particle size was observed to be around 20-40 nm with a height of 7-9 nm and approximate quantum yield of 0.23. The nanostructures were useful for bio imaging and cell tracking via fluorescence microscopy up to 12 generations with nominal cytotoxicity. The material was capable of scavenging free radicals from cellular microenvironment and downregulate gene expression of free radical scavenging enzymes. The material has significant relaxivity (r1) value of 3.98 mM(-1).sec(-1) at 1.5 T. It was also observed to create significant contrast with high circulation time (30 min) and renal clearance property. The histological analysis of kidney and liver sections were observed to have no significant toxicity from the nanostructure.