Pharmacokinetic and pharmacodynamic integration of enrofloxacin against Salmonella Enteritidis after administering to broiler chicken by per-oral and intravenous routes

摘要

It is crucial to optimize the dose of fluoroquinolones to avoid antibiotic resistance and to attain clinical success. We undertook this study to optimize the dose of enrofloxacin against Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) in chicken by assessing its pharmacokinetic/pharmacodynamic (PK/PD) indices. The antibacterial activities of enrofloxacin against S. Enteritidis were evaluated. After administering 10 mg/kg body weight (b.w.) of enrofloxacin to broiler chickens of both sexes by intravenous (IV) and peroral (PO) routes, blood samples were drawn at different intervals and enrofloxacin concentrations in plasma were determined. PK/PD indices were calculated by integrating the PK and PD data. The elimination half-lives (T-1/2), time required to reach peak concentration (T-max), peak concentration (C-max), and area under curve (AUC) after administering enrofloxacin by PO and IV routes were 25.84 +/- 1.40 h, 0.65 +/- 0.12 h, 3.82 +/- 0.59 mu g/mL, and 20.84 +/- 5.0 mu g.h/mL, and 12.84 +/- 1.4 h, 0.22 +/- 0.1 h, 6.74 +/- 0.03 mu g/mL, and 21.13 +/- 0.9 mu g.h/mL, respectively. The bioavailability of enrofloxacin was 98.6% +/- 8.9% after PO administration. The MICs of enrofloxacin were 0.0625-1 mu g/mL against S. Enteritidis strains, and the MIC50 was 0.50 mu g/mL. The C-max/MIC50 were 7.64 +/- 0.2 and 13.48 +/- 0.7 and the 24 h AUC/MIC50 were 41.68 +/- 0.1 and 42.26 +/- 0.3 after administering the drug through PO and IV routes, respectively. The data in this study indicate that the application of 50 mg/kg b.w. of enrofloxacin to chicken through PO and IV routes with a dosing interval of 24 h can effectively cure S. Enteritidis infection, indicating the need for a 5-fold increase in the recommended dosage of enrofloxacin in chicken.