首页> 外文期刊>Molecular ecology resources >Detecting alternatively spliced transcript isoforms from single‐molecule long‐read sequences without a reference genome

【24h】

Detecting alternatively spliced transcript isoforms from single‐molecule long‐read sequences without a reference genome

机译：在没有参考基因组的情况下检测来自单分子长读取序列的可选转录成像素

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Abstract > Alternative splicing ( <fc>AS</fc> ) is a major source of transcript and proteome diversity, but examining <fc>AS</fc> in species without well‐annotated reference genomes remains difficult. Research on both human and mouse has demonstrated the advantages of using Iso‐Seq? data for isoform‐level transcriptome analysis, including the study of <fc>AS</fc> and gene fusion. We applied Iso‐Seq? to investigate <fc>AS</fc> in Amborella trichopoda, a phylogenetically pivotal species that is sister to all other living angiosperms. Our data show that, compared with <fc>RNA</fc> ‐Seq data, the Iso‐Seq? platform provides better recovery on large transcripts, new gene locus identification and gene model correction. Reference‐based <fc>AS</fc> detection with Iso‐Seq? data identifies <fc>AS</fc> within a higher fraction of multi‐exonic genes than observed for published <fc>RNA</fc> ‐Seq analysis (45.8% vs. 37.5%). These data demonstrate that the Iso‐Seq? approach is useful for detecting <fc>AS</fc> events. Using the Iso‐Seq‐defined transcript collection in Amborella as a reference, we further describe a pipeline for detection of <fc>AS</fc> isoforms from PacBio Iso‐Seq? without using a reference sequence ( de novo ). Results using this pipeline show a 66%–76% overall success rate in identifying <fc>AS</fc> events. This de novo <fc>AS</fc> detection pipeline provides a method to accurately characterize and identify bona fide alternatively spliced transcripts in any nonmodel sys </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”xml：id =“men12670-abs-0001”> <标题类型=“main”>抽象</ title> < P>替代剪接（<Fc>为</ fc>）是转录物和蛋白质组多样性的主要来源，但在没有注释的参考基因组的物种中检查<Fc>作为</ Fc>仍然困难。对人类和小鼠的研究表明使用ISO-SEQ的优势？同种型分析的数据分析，包括<FC>作为</ Fc>和基因融合的研究。我们应用了ISO-SEQ？在 Amborella trichopoda中探讨<fc>作为</ fc>，</ i>是姐姐到所有其他生物的姐妹。我们的数据显示，与<FC> RNA相比，iso-seq（ISO-SEQ）相比？平台在大转录物中提供更好的恢复，新的基因座位识别和基因模型校正。基于参考的<FC>作为</ fc>检测，使用ISO-SEQ？数据在多偏振基因的较高分数内识别<Fc>作为</ Fc> RNA </ Fc> -SEQ分析（45.8％vs.37.5％）。这些数据表明ISO-SEQ？方法对于检测<FC>作为</ FC>事件有用。在 amborella </ i>中使用ISO-SEQ定义的转录物收集作为参考，我们进一步描述了从PACBIO ISO-SEQ检测<FC>作为同种型的管道？不使用参考序列（ de novo </ i>）。使用该管道的结果显示识别<FC>作为</ FC>事件时的总成功率为66％-76％。这 de novo </ i> <fc>作为</ fc>检测管道提供了一种准确表征和识别BONA在任何非典范系统中的拼接转录物的方法 </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-25257/'>《Molecular ecology resources》</a> <b style="margin: 0 2px;">|</b><span>2017年第6期</span><b style="margin: 0 2px;">|</b><span>共14页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liu Xiaoxian&option=202" target="_blank" rel="nofollow">Liu Xiaoxian;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mei Wenbin&option=202" target="_blank" rel="nofollow">Mei Wenbin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Soltis Pamela S.&option=202" target="_blank" rel="nofollow">Soltis Pamela S.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Soltis Douglas E.&option=202" target="_blank" rel="nofollow">Soltis Douglas E.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Barbazuk W. Brad&option=202" target="_blank" rel="nofollow">Barbazuk W. Brad;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of BiologyUniversity of FloridaGainesville FL 32611‐8525 USA;</p> <p>Department of BiologyUniversity of FloridaGainesville FL 32611‐8525 USA;</p> <p>Florida Museum of Natural HistoryUniversity of FloridaGainesville FL 32611‐7800 USA;</p> <p>Department of BiologyUniversity of FloridaGainesville FL 32611‐8525 USA;</p> <p>Department of BiologyUniversity of FloridaGainesville FL 32611‐8525 USA;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/179.html" title="分子生物学">分子生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=alternative splicing&option=203" rel="nofollow">alternative splicing;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Amborella trichopoda&option=203" rel="nofollow">Amborella trichopoda;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=pipeline&option=203" rel="nofollow">pipeline;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=single‐molecule long‐read sequencing&option=203" rel="nofollow">single‐molecule long‐read sequencing;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=transcriptomics&option=203" rel="nofollow">transcriptomics;</a> </p> <div class="translation"> 机译：替代拼接;Amborella Trichopoda;管道;单分子长读测序;转录组; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023742911.html">Detecting alternatively spliced transcript isoforms from single‐molecule long‐read sequences without a reference genome</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liu Xiaoxian&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Liu Xiaoxian,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mei Wenbin&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Mei Wenbin,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Soltis Pamela S.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Soltis Pamela S.,</a> <a href="/journal-foreign-25257/" target="_blank" rel="nofollow" class="tuijian_authcolor">Molecular ecology resources .</a> <span>2017</span><span>,第6期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：在没有参考基因组的情况下检测来自单分子长读取序列的可选转录成像素</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023791779.html">PacBio single-molecule long-read sequencing shed new light on the transcripts and splice isoforms of the perennial ryegrass</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xie Lijuan&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Xie Lijuan,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Teng Ke&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Teng Ke,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tan Penghui&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Tan Penghui,</a> <a href="/journal-foreign-25490/" target="_blank" rel="nofollow" class="tuijian_authcolor">Molecular genetics and genomics: MGG .</a> <span>2020</span><span>,第2期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：PacBio单分子长读测序棚新光线上的常年黑麦草的成绩单和剪接同种型</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704024534256.html">Analysis of transcripts and splice isoforms in Medicago sativa L. by single-molecule long-read sequencing</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chao Yuehui&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Chao Yuehui,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yuan Jianbo&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Yuan Jianbo,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guo Tao&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Guo Tao,</a> <a href="/journal-foreign-29495/" target="_blank" rel="nofollow" class="tuijian_authcolor">Plant Molecular Biology .</a> <span>2019</span><span>,第3期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用单分子长读测序分析Medicago Sativa L的转录同种型</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-226334_thesis/020516966809.html">DASE: Condition-specific differential alternative splicing variants estimation method without reference genome sequence, and its application to non-model organisms</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kouki Yonezawa&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Kouki Yonezawa,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tsukasa Mori&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Tsukasa Mori,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shuichi Shigeno&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Shuichi Shigeno,</a> <a href="/conference-foreign-226334/" target="_blank" rel="nofollow" class="tuijian_authcolor">IEEE International Conference on Bioinformatics and Biomedicine .</a> <span>2016</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：DASE：无参考基因组序列的条件特异性差异替代剪接变体估计方法，其在非模式生物中的应用</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/0206164397.html">Single Molecule, Long Read-based Methods for Studying Epigenetic Heterogeneity in Bacterial Genomes and Metagenomes</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Beaulaurier, John Anthony.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Beaulaurier, John Anthony. </a> <span>2018</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：单分子，基于长读取的方法研究细菌基因组和元基因组的表观遗传异质性</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040003267903.html">Analysis of transcripts and splice isoforms in red clover (Trifolium pratense L.) by single-molecule long-read sequencing</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yuehui Chao&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Yuehui Chao,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jianbo Yuan&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Jianbo Yuan,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sifeng Li&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Sifeng Li,</a> <span>2018</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：单分子长读测序分析红三叶草（Trifolium pratense L.）中的转录本和剪接同工型</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/01000131159398.html">Detecting alternatively spliced transcript isoforms from single‐molecule long‐read sequences without a reference genome</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xiaoxian Liu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Xiaoxian Liu,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wenbin Mei&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Wenbin Mei,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pamela S. Soltis&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Pamela S. Soltis,</a> <span>2017</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：在没有参考基因组的情况下检测来自单分子长读序列的可选转录转录物同种型</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-zhejiang-agriculture-forestry-university_thesis/0201290174444.html">毛竹长末端重复序列反转录转座子的全基因组特征及进化分析</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈娅欣&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 陈娅欣</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=周明兵&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,周明兵</a> <span> <a href="/journal-cn-55207/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 浙江农林大学学报 </a> </span> <span> . 2021</span><span>,第003期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-zhejiang-university_thesis/0201290443103.html">毛竹长末端重复序列反转录转座子的全基因组特征及进化分析</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈娅欣&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 陈娅欣</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=周明兵&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,周明兵</a> <span> <a href="/journal-cn-7269/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 浙江农林大学学报 </a> </span> <span> . 2021</span><span>,第003期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020315292268.html">基于长短读长和参考基因组的组装错误检测算法的研究</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=宋长进&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 宋长进</a> <span> . 2019</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120112374242.html">无参考基因组序列的转录组分析方法及系统</a> <b>[P]</b> . <span> 中国专利： CN112397149A </span> <span> . 2021-02-23</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130450055640.html">Differential determination of alternatively spliced RNAs, useful e.g. for detecting mutant receptor sequence associated with myeloid leukemia, by isoform-specific polymerase chain reaction</a> <b>[P]</b> . <span> 外国专利：  DE19854955A1 </span> <span> . 2000-05-31</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：鉴别可变剪接的RNA，例如亚型特异性聚合酶链反应检测与髓样白血病相关的突变受体序列 </span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130425404003.html">A METHOD TO DEVELOP A RELIABLE OLIGONUCLEOTIDE CHIP FOR PROFILING ALTERNATIVELY SPLICED TRANSCRIPTS FROM EUKARYOTIC GENOMES USING THE MICROARRAY TECHNOLOGY.</a> <b>[P]</b> . <span> 外国专利：  IN2009CH00457A </span> <span> . 2011-02-04</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：一种使用微阵列技术从真核基因组产生交替剪接的转录产物的可靠寡核苷酸芯片的方法。 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130418277983.html">ISOFORMS OF THE HUMAN SST5 RECEPTOR ORIGINATED BY ALTERNATIVE SPLICING AND OLIGONUCLEOTIDE PAIRS TO DETECT THEM BY PCR</a> <b>[P]</b> . <span> 外国专利：  US2014248628A1 </span> <span> . 2014-09-04</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：交替剪接产生的人类SST5受体的同工型和寡核苷酸对通过PCR检测它们 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704023742911','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche">  </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);">回到顶部</a> </li> <li class="shouye"> <a href="/">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html">关于掌桥</a></li> <li><a href="/help/helpmap.html">资源导航</a></li> <li><a href="/help/helpguide.html">新手指南</a></li> <li><a href="/help/helpcenter.html">常见问题</a></li> <li><a href="/sitemap.html">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704023742911down" data-source="7," data-out-id="1Bm1w90yZkoggXM+/Ddlzw==," data-f-source-id="7" data-title="Detecting alternatively spliced transcript isoforms from single‐molecule long‐read sequences without a reference genome" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="25257" id="journalid"> <input type="hidden" value="1" id="inyn_provide_service_level"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="Detecting alternatively spliced transcript isoforms from single‐molecule long‐read sequences without a reference genome"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.7.7"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.7.7"></script> </body> <script> $(function(){ var weiPuStatus = getCookie('WeiPuStatus'); if(weiPuStatus){ tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); var inyn_provide_service_level = $("#inyn_provide_service_level").val(); if((sourcetype ==1||sourcetype==4)&&inyn_provide_service_level!=4){ getJournal(); } }) </script> </html>