A fluorometric method for aptamer-based simultaneous determination of two kinds of the fusarium mycotoxins zearalenone and fumonisin B-1 making use of gold nanorods and upconversion nanoparticles

摘要

An aptamer-based assay for the determination of two different kinds of fusarium mycotoxins, i.e., zearalenone (ZEN) and fumonisin B-1 (FB1), is presented. Based on the inner filter effect (IFE) strategy, the contents of ZEN and FB1 can be simultaneously quantified. It is making use of 65-nm gold nanorods (AuNRs), 20-nm upconversion nanoparticles (UCNPs), fluorescence dyes, and DNA sequences. In the absence of ZEN and FB1, the UCNPs and AuNRs associate through DNA sequences. Due to IFE effect, weak fluorescence signals are collected. In the presence of ZEN or FB1, UCNPs and AuNRs become unstable and partially separate from each other. This results in the recovery of fluorescence signals. Under 980-nm laser excitation, the logarithmic values of fluorescence signal intensities at 606 nm and 753 nm gradually increase with the concentration of ZEN and FB1 in the ranges 0.05-100 mu g L-1 (the coefficient of determination is 0.997) and 0.01-100 ng L-1 (the coefficient of determination is 0.986), respectively. The limits of detection (LOD) of the fabricated assay for ZEN and FB1 are 0.01 mu g L-1 and 0.003 ng L-1, respectively. The proposed method has a high selectivity over other competitive mycotoxins, including aflatoxin B1, ochratoxin A, patulin and ochratoxin B. The applicability of the assay was evaluated in the determination of ZEN and FB1 contents in spiked corn samples. The average recoveries ranged from 89.9 to 106.6%. This result confirms the practicality of this method.