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首页> 外文期刊>Microbial drug resistance: MDR : Mechanisms, epidemiology, and disease >Iodometric and Molecular Detection of ESBL Production Among Clinical Isolates of E-coli Fingerprinted by ERIC-PCR: The First Egyptian Report Declares the Emergence of E-coli O25b-ST131clone Harboring bla(GES)
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Iodometric and Molecular Detection of ESBL Production Among Clinical Isolates of E-coli Fingerprinted by ERIC-PCR: The First Egyptian Report Declares the Emergence of E-coli O25b-ST131clone Harboring bla(GES)

机译：ERIC-PCR临床分离素临床分离株的ISBL型和分子检测：第一埃及报告宣布E-COLI O25B-ST131Clone窝藏BLA（GES）的出现

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The extensive use of beta-lactam antibiotics has led to emergence and spread of extended-spectrum beta-lactamases (ESBLs). This study was conducted to investigate the prevalence of 7 different ESBL genes (bla(TEM), bla(SHV), bla(CTX-M), bla(VEB), bla(PER), bla(GES), and bla(OXA)-10) and O25b-ST131 high-risk clone among 61 clinical isolates of Escherichia coli. Also, one broad-spectrum beta-lactamase (bla(OXA-1)) was investigated. This study was also constructed to evaluate iodometric overlay method in detection of ESBL production. Phenotypic identification of E. coli isolates using API 20E revealed 18 distinct biotypes. DNA fingerprinting using enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) differentiated all isolates into 2 main phylogenetic groups with 60 distinct genetic profiles. Elevated values of minimal inhibitory concentration (MIC)(50) and MIC90 for third-and fourth-generation cephalosporins were observed. Phenotypic tests revealed that 85.24% of isolates were ESBL producers. The incidence rates of bla(TEM), bla(SHV), bla(CTX-M), bla(GES), bla(OXA-1), and bla(OXA-10) among E. coli ESBL producer phenotype were 69.23%, 25%, 96.15%, 3.85%, 11.54%, and 48%, respectively. On the other hand, bla(VEB) and bla(PER) were not detected. Sequencing of bla(TEM) and bla(SHV) revealed that bla(TEM-214) and blaSHV-11 were the most prevalent variants. Group characterization of bla(CTX-M) revealed that bla(CTX-M-1) was the most prevalent group of bla(CTX-M) family. It was found that 30.77% of E. coli ESBL producers belonged to O25bST131 clone harboring bla(CTX-M-15). This study concluded that iodometric overlay method was 100% sensitive in detection of ESBL production. To our knowledge, this is the first Egyptian study that declares the emergence of E. coli O25b-ST131 harboring bla(GES).
机译：β-内酰胺抗生素的广泛使用导致扩展光谱β-内酰胺酶（ESBLS）的出现和扩散。进行该研究以研究7种不同ESBL基因的患病率（BLA（TEM），BLA（SHV），BLA（CTX-M），BLA（VEB），BLA（PER），BLA（GES）和BLA（OXA） ）-10）和O25B-ST131高风险克隆在大肠杆菌的61个临床分离物中。此外，研究了一种广谱β-内酰胺酶（BLA（OXA-1））。该研究还构造成评估在检测ESBL生产中的碘覆覆盖方法。使用API​​ 20e的大肠杆菌分离株的表型鉴定揭示了18个不同的生物型。使用肠杆菌重复性代号的DNA指纹识别聚合酶链反应（ERIC-PCR）将所有分离物分化为2个主要的系统发育基团，其中60个不同的遗传谱。观察到第三代和第四代头孢菌素的最小抑制浓度（MIC）（50）和MIC90的升高值。表型试验显示，85.24％的分离物是ESBL生产商。 BLA（TEM），BLA（SHV），BLA（CTX-M），BLA（GES），BLA（OXA-1）和BLA（OXA-10）的发病率为69.23％分别为25％，96.15％，3.85％，11.54％和48％。另一方面，未检测到BLA（VEB）和BLA（PER）。 BLA（TEM）和BLA（SHV）的测序揭示了BLA（TEM-214）和Blashv-11是最普遍的变体。 BLA（CTX-M）的组特征揭示了BLA（CTX-M-1）是最普遍的BLA（CTX-M）家族。发现30.77％的大肠杆菌ESBL生产者属于O25BST131克隆含BLA（CTX-M-15）。该研究得出结论，在检测ESBL生产中，碘量覆盖方法是100％敏感性。为了我们的知识，这是第一项埃及研究，宣布窝藏BLA（GES）的大肠杆菌O25B-ST131的出现。

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