In Vitro and In Vivo Antimicrobial Activity of Cymbopogon citratus (DC.) Stapf. Against Staphylococcus spp. Isolated from Newborn Babies in an Intensive Care Unit

摘要

Phytotherapy is an emerging topic of health research, with particular focus on studying the efficiency of essential oils as antimicrobials. In this study, we investigated the in vitro and in vivo susceptibility of Staphylococcus strains isolated from newborns to Cymbopogon citratus oil. The in vitro susceptibility of the microorganisms to C. citratus essential oil was compared with the activities of standard antibiotics administered to newborns using disk diffusion and microdilution methods. For in vivo assessment, 30 Wistar rats were wounded and subjected to infection of Staphylococcus aureus strain DRJ080, followed by treatment with the antibiotic vancomycin, C. citratus, or carbopol polymer gel (control) for 11 days. S. aureus accounted for 23.36% of the 107 Staphylococcus sp. strains isolated. Both vancomycin and the essential oil of C. citratus inhibited the growth of all microorganisms in vitro. The minimum inhibitory and bactericidal concentrations for the C. citratus oil were 0.625?mg/mL in all strains tested. The oil had the same therapeutic effectiveness as vancomycin against S. aureus DRJ080 in rats. Thus, Staphylococcus strains of newborns are sensitive to C. citratus oil, both in vitro and in vivo, demonstrating its potential as an antibiotic alternative.