A new method for spectrophotometric determination of carbaryl based on rubber tree bark peroxidase enzymatic reaction

摘要

A method for the determination of carbaryl in vegetable samples was developed employing peroxidase enzyme extracts from newly excised rubber tree bark strips as a catalyst. The solid phase extraction method QuEChERS was utilized for sample preparation followed by dispersive liquid-liquid microextraction (DLLME) to increase the concentration of the analyte before analysis by UV-Visible spectrophotometry. Parameters influencing this enzymatic development method and efficiency of DLLME were carefully studied. Under selected conditions, absorbance was in proportion to carbaryl concentration in the range of 0.1-3.0 mg L-1 with linear regression (r(2)) of 0.9999. Limit of detection (LOD) and limit of quantitation (LOQ) were 0.06 mg L-1 and 0.25 mg L-1, respectively. Relative standard deviation (RSD) values for the samples were 2.51-4.06% (n = 7). Good recoveries of carbaryl were obtained in the range of 83-118%. The proposed method proved rapid, reliable and selective for determination of carbaryl in vegetable samples.