A simple and label-free genosensor for BRCA1 related sequence based on electrospinned ribbon conductive nanofibers

摘要

The Breast cancer is the second most common cancer in women. So early detection of cancer is very important. We prepared a novel electrochemical genosensor for label-free, sensitive and selective detection of BRCA1 gene sequence. The developed sensor capture probe (DNA-p) hybridize to target BRCA1 gene sequence (DNA-t) on electrospinned ribbon conductive nanofibers (RCNFs) nanofiber electrospinned ribbon conductive nanofibers of polyethersulfone and nanotubes (RCNFs - MWCNTs) modified carbon paste electrode. This platform has a conductive and high mechanical strength and the surface to electrode volume ratio has increased 2 times. The construction of the platform for the sensor is very simple and cost-effective and the nanofiber can be spun in one step on the electrode surface. The BRCA1 gene sequence could be specifically recognized by capture aptamer pre-immobilized on modified carbon paste surface. Under optimum conditions, the electrochemical impedance spectroscopy (EIS) signals were proportional to the BRCA1 gene sequence concentration from 5 to 14,000 pM with a detection limit of 2.4 pM. The genosensor also exhibited high selectivity, stability and reproducibility with recoveries between 101.5 and 105.2%, indicating the developed biosensor would be a potential alternative tool for BRCA1 gene sequence detection in real biological samples.