Population genomic SNPs from epigenetic RADs: Gaining genetic and epigenetic data from a single established next-generation sequencing approach

摘要

Epigenetics is increasingly recognized as an important molecular mechanism underlying phenotypic variation. To study DNA methylation in ecological and evolutionary contexts, epiRADseq is a cost-effective next-generation sequencing (NGS) technique based on reduced representation sequencing of genomic regions surrounding non-/methylated sites. EpiRADseq for genome-wide methylation abundance and ddRADseq for genome-wide single-nucleotide polymorphism (SNP) genotyping follow very similar library and sequencing protocols, but to date these two types of dataset have been handled separately. Here we test the performance of using epiRADseq data to generate SNPs for population genomic analyses.