Decalepis hamiltonii protects H9c2 cells against H_2O_2 induced oxidative stress and apoptosis through modulating antioxidant status

摘要

Decalepis hamiltonii (Dh) is used to treat various diseases in Indian traditional medicine due to its health-promoting properties. However, the cardioprotective effect of Dh against oxidative stress remains unclear. In the present study, we investigated the protective effect of Dh on H2O2 induced oxidative stress in H9c2 cells. Pre-treatment with Dh extract prior to H_2O_2 exposure significantly increased the cell viability as measured by MTT assay and decreased the levels of LDH release. Dh extractinhibited H2O2 induced apoptosis and enhanced the number of viable cells in comparison to H_2O_2 treated cells as revealed by Flow cytometry analysis, AO/EB and DAPI staining. Furthermore, the Dh extract attenuated the H2O2 induced oxidative stress by up-regulating the antioxidant enzymes, reducing oxidative stress markers and inhibiting ROS generation. These results demonstrate that Dh extract ameliorates H_2O_2 induced cytotoxicity and may be a promising therapeutic intervention in treating oxidativestress related cardiovascular diseases.