Based on magnetic beads to develop the kit for extraction of high-quality cell-free DNA from blood of breast cancer

摘要

Early detection of breast cancer (BC) is very important to avoid mortality caused by it. It is concluded in recent studies that the DNA in patient's serum can be used as biomarker for cancer detection. Therefore, extracting high-quality DNA from patient's serum can achieve this goal. In this work, we compared three methods (phenol-chloroform, column-based and magnetic beads) of extracting DNA from serum by means of purchased commercial kits and concluded that column-based kit is the best one to extract Cell-free DNA (cfDNA). Then we developed a new kit named PuriMag magnetic beads kit to extract DNA from serum of BC patients. Further, we described the properties of this new kit including its recovery capacity and stability. We compared the extraction performances of PuriMag magnetic beads kit and column-based kit and concluded that PuriMag magnetic beads are a better method to extract cfDNA. Surely PuriMag magnetic beads also suit automation and will be a useful method for fast, high-quality and automatic DNA extraction from serum.