Droplet‐Based Microfluidic Templating of Polyglycerol‐Based Microgels for the Encapsulation of Cells: A Comparative Study

Kapourani Era; Neumann Falko; Achazi Katharina; Dernedde Jens; Haag Rainer

摘要

Abstract >Cell microencapsulation holds great promise as a therapeutic strategy for the controlled and sustained delivery of biologically relevant agents. The authors developed cell‐laden microgel scaffolds with excellent long‐term viabilities by combining bioorthogonal strain promoted azide–alkyne cycloaddition (SPAAC) and droplet‐based microfluidic templating. Star‐shaped polyglycerol hexaazide, α,ω‐bis azido‐linear polyglycerol or polyethylene glycol as well as dendritic polyglycerol‐(polycyclooctyne) served as bioinert hydrogel precursors. The authors demonstrate for the first time the generation of entirely polyglycerol‐based microcapsules with excellent stability and full retention of viability of the packed cells for longer than 3 weeks. As a result, our microgel particles could be used for long‐term immunoisolation of cells enabling their study during encapsulation. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >细胞微胶囊占据了对生物学相关代理的受控和持续交付的治疗策略。作者通过组合生物正常菌株促进的叠氮化物 - 炔环加湿（SPAAC）和基于液滴的微流体模板，开发了具有优异的长期活力的细胞载花粉微凝胶支架。星形聚甘油六氮杂物，α，ω-Bisα-线性聚甘油或聚乙二醇以及树枝状聚甘油 - （多环胶苷元）作为生物素蛋白前体。作者首次证明了完全聚甘油基微胶囊的产生具有优异的稳定性和填充细胞的存活率的完全保留时间超过3周。结果，我们的微凝胶颗粒可用于长期免疫分离的细胞，使其在封装期间进行研究。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-24307/'>《Macromolecular bioscience》</a> <b style="margin: 0 2px;">|</b><span>2018年第10期</span><b style="margin: 0 2px;">|</b><span>共10页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kapourani Era&option=202" target="_blank" rel="nofollow">Kapourani Era;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Neumann Falko&option=202" target="_blank" rel="nofollow">Neumann Falko;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Achazi Katharina&option=202" target="_blank" rel="nofollow">Achazi Katharina;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Dernedde Jens&option=202" target="_blank" rel="nofollow">Dernedde Jens;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Haag Rainer&option=202" target="_blank" rel="nofollow">Haag Rainer;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Freie Universit?t BerlinTakustrasse 3 14195 Berlin Germany;</p> <p>Freie Universit?t BerlinTakustrasse 3 14195 Berlin Germany;</p> <p>Freie Universit?t BerlinTakustrasse 3 14195 Berlin Germany;</p> <p>Charité–Universit?tsmedizin Berlin corporate member of Freie Universit?t Berlin Humboldt‐Universit?t zu Berlin and Berlin Institute of HealthInstitut für Laboratoriumsmedizin Klinische Chemie und Pathobiochemie CVKAugustenburger Platz 1 13353 Berlin;</p> <p>Freie Universit?t BerlinTakustrasse 3 14195 Berlin Germany;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1185.html" title="高分子化学（高聚物）">高分子化学（高聚物）;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=cell encapsulation therapies&option=203" rel="nofollow">cell encapsulation therapies;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=dendritic polyglycerol&option=203" rel="nofollow">dendritic polyglycerol;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=droplet‐based microfluidics&option=203" rel="nofollow">droplet‐based microfluidics;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=microgels&option=203" rel="nofollow">microgels;</a> </p> <div class="translation"> 机译：细胞包封疗法;树突式聚甘油;基于液滴的微流体;微凝胶; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023543212.html">Droplet‐Based Microfluidic Templating of Polyglycerol‐Based Microgels for the Encapsulation of Cells: A Comparative Study</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kapourani Era&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Kapourani Era,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Neumann Falko&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Neumann Falko,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Achazi Katharina&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Achazi Katharina,</a> <a href="/journal-foreign-24307/" target="_blank" rel="nofollow" class="tuijian_authcolor">Macromolecular bioscience .</a> <span>2018</span><span>,第10期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于液体基微流体模板的聚甘油基微凝胶，用于封装细胞：比较研究</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-foreign_detail_thesis/0204116060007.html">Parallel droplet microfluidics for high throughput cell encapsulation and synthetic microgel generation</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Devon M. Headen&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Devon M. Headen,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=José R. García&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">José R. García,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Andrés J. García&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Andrés J. García </a> <a href="/journal-foreign-24954/" target="_blank" rel="nofollow" class="tuijian_authcolor">Microsystems & Nanoengineering .</a> <span>2018</span><span>,第1期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：平行液滴微流控技术用于高通量细胞包封和合成微凝胶生成</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-foreign_advanced-materials_thesis/020411849344.html">Microfluidic-Based Generation of Size-Controlled, Biofunctionalized Synthetic Polymer Microgels for Cell Encapsulation</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Devon M. Headen&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Devon M. Headen,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guillaume Aubry&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Guillaume Aubry,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hang Lu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Hang Lu,</a> <a href="/journal-foreign-1128/" target="_blank" rel="nofollow" class="tuijian_authcolor">Advanced Materials .</a> <span>2014</span><span>,第19期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于微流控的尺寸控制，生物功能化合成聚合物微凝胶用于细胞封装</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-241697_thesis/020517210122.html">3D Printing for Microgel-Based Liver Cell Encapsulation</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jonathan S. O’Connor&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Jonathan S. O’Connor,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Heesoo Kim&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Heesoo Kim,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Eunheui Gwag&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Eunheui Gwag,</a> <a href="/conference-foreign-241697/" target="_blank" rel="nofollow" class="tuijian_authcolor">IEEE International Conference on Micro Electro Mechanical Systems .</a> <span>2021</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用于微凝胶基肝细胞封装的3D打印</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/0206125463.html">Three-dimensional microfluidic based multicellular model for studying cancer metastases.</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Huang, Carlos Pablo.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Huang, Carlos Pablo. </a> <span>2010</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于三维微流控的多细胞模型，用于研究癌症转移。</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040005649607.html">Microfluidic-Based Generation of Size-Controlled Biofunctionalized Synthetic Polymer Microgels for Cell Encapsulation</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Devon M. Headen&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Devon M. Headen,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guillaume Aubry&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Guillaume Aubry,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hang Lu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Hang Lu,</a> <span>-1</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于微流控的尺寸控制生物功能化合成聚合物微凝胶用于细胞封装</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/0100048859520.html">Droplet-Based Microfluidic Platforms for the Encapsulation and Screening of Mammalian Cells and Multicellular Organisms</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Clausell-Tormos Jenifer&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Clausell-Tormos Jenifer,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lieber Diana&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Lieber Diana,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Baret Jean-Christophe&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Baret Jean-Christophe,</a> <span>2008</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于液滴的微流控平台，用于封装和筛选哺乳动物细胞和多细胞生物</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-tissue-engineering-research_thesis/0201252239470.html">海马神经元条件培养液体外诱导大鼠骨髓间质干细胞向神经元样细胞和神经胶质样细胞的分化:与含b-FGF培养基和无血清培养基的比较</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李朝晖&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 李朝晖</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=蔡志平&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,蔡志平</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=崔慧先&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,崔慧先</a> <span> <a href="/journal-cn-15352/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国组织工程研究 </a> </span> <span> . 2010</span><span>,第006期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-qingdao-university-engineering-technology-edition_thesis/0201210139838.html">双功能化、双重响应聚(N-异丙基丙烯酰胺)基微凝胶的研究</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李艳红&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 李艳红</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=马晓梅&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,马晓梅</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mitch Winnik&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Mitch Winnik</a> <span> <a href="/journal-cn-7577/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 青岛大学学报（工程技术版） </a> </span> <span> . 2011</span><span>,第004期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-xiangnan-university-medical-sciences_thesis/0201246418667.html">液基薄层细胞学与巴氏涂片法用于宫颈细胞学的比较研究</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=罗建湘&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 罗建湘</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=谢聪莉&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,谢聪莉</a> <span> <a href="/journal-cn-6563/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 湘南学院学报（医学版） </a> </span> <span> . 2006</span><span>,第004期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-family-planning_thesis/0201212653915.html">传统宫颈细胞学与液基薄层细胞/TBS检测方法用于宫颈病变早期筛查的比较研究</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=冯睿&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 冯睿</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王倩&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,王倩</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=邱新娇&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,邱新娇</a> <span> <a href="/journal-cn-5578/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国计划生育学杂志 </a> </span> <span> . 2004</span><span>,第011期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_seed-science-technology_thesis/0201291693868.html">纸桥法液体培养基替代琼脂固体培养基用于食用菌菌种培养的初步探索</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=栗志&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 栗志</a> <span> <a href="/journal-cn-6864/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 种子科技 </a> </span> <span> . 2021</span><span>,第020期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-43337_thesis/020222760286.html">微流控技术制备用于三维细胞培养的双交联透明质酸基微凝胶</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李文秀&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 李文秀</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=马婷&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,马婷</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=曹晓东&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,曹晓东</a> <span> <a href="/conference-cn-43337/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 2017中国生物材料大会 </a> <span> <span> . 2017</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020313014139.html">基于聚缩水甘油的靶向型钆基大分子MRI造影剂的制备与性能研究</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=曹翼&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 曹翼</a> <span> . 2017</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/061201234701.html">二缩水甘油基异氰尿酸基改性有机聚硅氧烷以及含有该有机聚硅氧烷的组成物</a> <b>[P]</b> . <span> 中国专利： CN101538367B </span> <span> . 2013.09.04</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120105744210.html">二缩水甘油基异氰尿酸基改性有机聚硅氧烷以及含有该有机聚硅氧烷的组成物</a> <b>[P]</b> . <span> 中国专利： CN101538367A </span> <span> . 2009-09-23</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130409152326.html">DROPLET-BASED CELL CULTURE AND CELL ASSAYS USING DIGITAL MICROFLUIDICS</a> <b>[P]</b> . <span> 外国专利：  CA2639954C </span> <span> . 2017-08-15</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于数字微流控技术的基于液滴的细胞培养和细胞评估 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130418068471.html">Droplet-based cell culture and cell assays using digital microfluidics</a> <b>[P]</b> . <span> 外国专利：  US8728291B2 </span> <span> . 2014-05-20</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于液滴的细胞培养和使用数字微流控技术的细胞测定 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130420403826.html">Droplet-based cell culture and cell assays using digital microfluidics</a> <b>[P]</b> . <span> 外国专利：  US8367370B2 </span> <span> . 2013-02-05</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于液滴的细胞培养和使用数字微流控技术的细胞测定 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704023543212','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="关闭" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche">  </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)" title="意见反馈">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);" title="回到顶部">回到顶部</a> </li> <li class="shouye"> <a href="/" title="首页">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html" title="关于掌桥">关于掌桥</a></li> <li><a href="/help/helpmap.html" title="资源导航">资源导航</a></li> <li><a href="/help/helpguide.html" title="新手指南">新手指南</a></li> <li><a href="/help/helpcenter.html" title="常见问题">常见问题</a></li> <li><a href="/sitemap.html" title="网站地图">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9" title="版权声明">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank" title="ICP备案号">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704023543212down" data-source="7," data-out-id="OmH6YvHosvaCgZH3Ia9gN9AKkzS5I4iSwnhcOrbiokYGhJPCw8WRFtXP/dHEKOJe," data-f-source-id="7" data-title="Droplet‐Based Microfluidic Templating of Polyglycerol‐Based Microgels for the Encapsulation of Cells: A Comparative Study" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="24307" id="journalid"> <input type="hidden" value="1" id="inyn_provide_service_level"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="Droplet‐Based Microfluidic Templating of Polyglycerol‐Based Microgels for the Encapsulation of Cells: A Comparative Study"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/newmail.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.7.8"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/sse.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.7.8"></script> </body> <script> $(function(){ var weiPuStatus = getCookie('WeiPuStatus'); if(weiPuStatus){ tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); var inyn_provide_service_level = $("#inyn_provide_service_level").val(); if((sourcetype ==1||sourcetype==4)&&inyn_provide_service_level!=4){ getJournal(); } }) </script> </html>