Role of Skin Layers on Mechanical Properties and Supercontraction of Spider Dragline Silk Fiber

Yazawa Kenjiro; Malay Ali D.; Masunaga Hiroyasu; Numata Keiji

摘要

Abstract >Spider dragline silk is a composite biopolymer that harbors extraordinary mechanical characteristics, and consists of a hierarchically arranged protein core surrounded by outer “skin” layers. However, the contribution of the successive fiber layers on material properties has not been well defined. Here, the influence of the different components on the physicochemical and mechanical properties of dragline is investigated. The crystal structure and the mechanical properties are not changed significantly after the removal of skin constituents, indicating that the core region of dragline silk fibers determines the structural and mechanical properties. Furthermore, the outer layers have little influence on supercontraction, suggesting they do not function as protection against the penetration of water molecules. On the other hand, the outer layers offer some protection against protease digestion. The present study provides insight into how the function and structure of silk fibers are modulated and facilitates the design of silk‐inspired functional materials. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >蜘蛛龙丝是一个复合生物聚合物，其竖立了非凡的机械特性，并且由分层排列而组成由外部“皮肤”层包围的蛋白质核心。然而，连续纤维层对材料特性的贡献尚未明确定义。这里，研究了不同组分对龙骨菌和力学性能的影响。在去除皮肤成分后，晶体结构和机械性能不会显着变化，表明拖线丝纤维的核心区域决定了结构和机械性能。此外，外层对超级共同几乎没有影响，表明它们不起作用以防止水分子的渗透。另一方面，外层对蛋白酶消化提供一些保护。本研究提供了熟悉丝纤维的功能和结构如何调制和促进丝绸启发功能材料的设计。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-24307/'>《Macromolecular bioscience》</a> <b style="margin: 0 2px;">|</b><span>2019年第3期</span><b style="margin: 0 2px;">|</b><span>共7页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yazawa Kenjiro&option=202" target="_blank" rel="nofollow">Yazawa Kenjiro;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Malay Ali D.&option=202" target="_blank" rel="nofollow">Malay Ali D.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Masunaga Hiroyasu&option=202" target="_blank" rel="nofollow">Masunaga Hiroyasu;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Numata Keiji&option=202" target="_blank" rel="nofollow">Numata Keiji;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Biomacromolecules Research TeamRIKEN Center for Sustainable Resource Science2‐1 Hirosawa Wako‐shi Saitama 351‐0198 Japan;</p> <p>Biomacromolecules Research TeamRIKEN Center for Sustainable Resource Science2‐1 Hirosawa Wako‐shi Saitama 351‐0198 Japan;</p> <p>Japan Synchrotron Radiation Research Institute1‐1‐1 Kouto Sayo‐cho Sayo‐gun Hyogo 679–5198 Japan;</p> <p>Biomacromolecules Research TeamRIKEN Center for Sustainable Resource Science2‐1 Hirosawa Wako‐shi Saitama 351‐0198 Japan;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1185.html" title="高分子化学（高聚物）">高分子化学（高聚物）;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=hierarchical organizations&option=203" rel="nofollow">hierarchical organizations;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=protease digestions&option=203" rel="nofollow">protease digestions;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=skin‐core structures&option=203" rel="nofollow">skin‐core structures;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=spider dragline silks&option=203" rel="nofollow">spider dragline silks;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=supercontraction&option=203" rel="nofollow">supercontraction;</a> </p> <div class="translation"> 机译：等级组织;蛋白酶消化;皮肤核心结构;蜘蛛拖拉线丝绒;超级共同; 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href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=周镜桂&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 周镜桂</a> <span> . 2014</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/061201640685.html">高分子量重组丝或类丝蛋白、以及使用它们制造的微米或纳米级蜘蛛丝或者类蜘蛛丝纤维</a> <b>[P]</b> . <span> 中国专利： CN102906107B </span> <span> . 2015.05.20</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120101706235.html">高分子量重组丝或类丝蛋白、以及使用它们制造的微米或纳米级蜘蛛丝或者类蜘蛛丝纤维</a> <b>[P]</b> . <span> 中国专利： CN102906107A </span> <span> . 2013-01-30</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130412252086.html">chimeric spider silk polypeptide, composite fiber, transgenic silkworm, nucleic acid encoding a chimeric spider silk polypeptide, piggybac vector, method for producing a chimeric spider silk fiber and method for preparing a silkworm transgenic bombyx mori silk capable of stably expressing a chimeric spider silk polypeptide suitable for mounting on a chimeric spider silk fiber</a> <b>[P]</b> . <span> 外国专利：  BR112013007247A2 </span> <span> . 2016-06-14</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：嵌合蜘蛛丝多肽，复合纤维，转基因蚕，编码嵌合蜘蛛丝多肽的核酸，piggybac载体，生产嵌合蜘蛛丝纤维的方法和制备能够稳定表达嵌合蜘蛛丝多肽的蚕转基因虫卵。适用于安装在嵌合蜘蛛丝纤维上 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130419711367.html">HIGH MOLECULAR WEIGHT RECOMBINANT SILK OR SILK-LIKE PROTEIN, AND MICRO- OR NANOSCALE SPIDER-WEB FIBER OR SPIDER-WEB-LIKE FIBER PRODUCED USING THE RECOMBINANT SILK OR SILK-LIKE PROTEIN</a> <b>[P]</b> . <span> 外国专利：  EP2546263A4 </span> <span> . 2013-07-31</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：高分子量重组真丝或类似丝蛋白，以及使用重组真丝或类似丝蛋白生产的微或纳米级蜘蛛网纤维或蜘蛛网状纤维 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130419711368.html">HIGH MOLECULAR WEIGHT RECOMBINANT SILK OR SILK-LIKE PROTEIN, AND MICRO- OR NANOSCALE SPIDER-WEB FIBER OR SPIDER-WEB-LIKE FIBER PRODUCED USING THE RECOMBINANT SILK OR SILK-LIKE PROTEIN</a> <b>[P]</b> . <span> 外国专利：  EP2546263A2 </span> <span> . 2013-01-16</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：高分子量重组真丝或类似丝蛋白，以及使用重组真丝或类似丝蛋白生产的微或纳米级蜘蛛网纤维或蜘蛛网状纤维 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 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