Determination of Major Phenolics Content in Dried Apples from Three New Cultivars (Malus domestica Borkh.) Using HPLC-UV-FL with Pentafluorophenyl Stationary Phase

摘要

The analysis of selected phenolic compounds in dried apple pulps with pentafluorophenyl (PFP) reversed-phase HPLC-UV-FL is presented. Three new apple cultivars named Nicoter Kanzi (R), Shinano Gold yello (R), and the red-fleshed one Luresweet Redlove (R) grown in one site in South Tyrol (Italy) have been investigated and compared with cv. Golden Delicious. Phenolics belonging to different classes (hydroxybenzoic acids, flavan-3-ols, caffeoylquinic derivatives, flavonols, and dihydrochalcones) have been analyzed. Extraction with aqueous methanolic mixture yielded good analytical recovery. Luresweet Redlove (R) showed the highest content of quercetin-3-O-glucoside, gallic acid, and chlorogenic acid and the lowest content of catechins and procyanidins; on the other hand, Nicoter Kanzi (R) showed the lowest concentration of quercetin-3-O-glucoside. The analytical procedure described is an easy and affordable methodology to investigate other apple varieties from local cultivations, to study their phytochemical composition, and to determine their potential nutraceutical values. The content of phenolic compounds in the three apple varieties Nicoter Kanzi (R), Shinano Gold yello (R), and Luresweet Redlove (R) was reported for the first time.