Quorum Sensing molecules produced by Pseudomonas aeruginosa impair attachment and biofilm formation in Candida albicans

摘要

Cell-to cell signaling represents maybe the most important route to control microbial behavior, virulence and infection. Although well investigated, the molecular mechanisms of Quorum Sensing (QS) communication is far of being completely understood, especially during inter-species and inter-kingdom communication. In this paper we investigated the interaction between the opportunistic pathogen Pseudomonas aeruginosa and Candida albicans empathizing on the impact of Pseudomonas aeruginosa main QS signaling molecules on attachment and biofilm formation of this yeast. Our results demonstrated that, among all tested molecules, N-(3-oxododecanoyl) homoserine lactone (OdDHL), 2-heptyl-4-quinolone (HHQ) and C4-HSL (N-butyryl-L-homoserine lactone) have the most significant modulatory properties regarding the attachment and biofilm formation of C. albicans. Moreover, the tested purified QS molecules but also P. aeruginosa supernatant (SN) proved to modulate in vitro attachment to cellular substrata in C. albicans.