Active Ingredients of Epimedii Folium and Ligustri Lucidi Fructus Balanced GR/HSP90 to Improve the Sensitivity of Asthmatic Rats to Budesonide

摘要

This study aimed to investigate the possible molecular mechanisms of active ingredients of Epimedii Folium (EF) and Ligustri Lucidi Fructus (LLF) combined with Budesonide (Bun) in asthmatic rats. Rats were divided into 5 groups, including normal group, asthma model group, Bun group, group of active ingredients of EL and LLF (EL), and group of coadministration of Bun with EL (Bun&EL). The asthmatic model was prepared by ovalbumin sensitizing and challenging. Lymphocyte apoptosis, GR protein and binding, and the protein and mRNA of GR alpha, GR beta, and HSP90 were tested. The results showed that Bun&EL (1) markedly increased lymphocyte apoptosis, GR and HSP90 protein, and GR binding in BALF and (2) enhanced the expressions of GR alpha and HSP90 and the ratio of GR alpha to GR beta to HSP90 both in protein and in mRNA levels in lung, (3) while decrease occurred in GR beta. mRNA and the mRNA ratio of GR beta to HSP90 compared with asthma or Bun group. Moreover, there was a significant correlation between GR alpha and GR beta in protein level, or between GR alpha and HSP90 both in protein and in mRNA levels. EL may effectively enhance the sensitivity of asthmatic rats to Bun via balancing GR/HSP90. And these findings will be beneficial for the treatment of asthma in the future.