Transcriptomic and physiological analyses of the dinoflagellate <i xmlns='http://www.wiley.com/namespaces/wiley'>Karenia mikimotoiKarenia mikimotoi reveal non‐alkaline phosphatase‐based molecular machinery of ATP utilisation

Luo Hao; Lin Xin; Li Ling; Lin Lingxiao; Zhang Chao; Lin Senjie

摘要

Summary > The ability to utilize dissolved organic phosphorus (DOP) is important for phytoplankton to survive the scarcity of dissolved inorganic phosphorus (DIP), and alkaline phosphatase (AP) has been the major research focus as a facilitating mechanism. Here, we employed a unique molecular ecological approach and conducted a broader search for underpinning molecular mechanisms of adenosine triphosphate (ATP) utilisation. Cultures of the dinoflagellate Karenia mikimotoi were set up in L1 medium (+P), DIP‐depleted L1 medium (–P) and ATP‐replacing‐DIP medium (ATP). Differential gene expression was profiled for ATP and +P cultures using suppression subtractive hybridisation (SSH) followed by 454 pyrosequencing, and RT‐qPCR methods. We found that ATP supported a similar growth rate and cell yield as L1 medium and observed DIP release from ATP into the medium, suggesting that K. mikimotoi cells were expressing extracellular hydrolases to hydrolyse ATP. However, our SSH, qPCR and enzymatic activity assays indicated that 5′‐nucleotidase (5NT), rather than AP, was responsible for ATP hydrolysis. Further gene expression analyses uncovered that intercellular purine metabolism was significantly changed following the utilisation of ATP. Our findings reveal a multi‐faceted machinery regulating ATP utilisation and P metabolism in K. mikimotoi , and underscore AP activity is not the exclusive indicator of DOP utilisation. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”> <title type =“main”>摘要</ title> >利用溶解有机磷的能力（DOP ）对于浮游植物来生存溶解无机磷（浸渍）的稀缺性是重要的，并且碱性磷酸酶（AP）是主要的研究重点作为促进机制。在这里，我们采用独特的分子生态学方法，并对腺苷三磷酸腺苷（ATP）利用的分子机制进行了更广泛的搜索。在L1培养基（+ P），浸渍耗尽的L1培养基（-P）和ATP替代浸渍培养基（ATP）中设立了丁诺蛋白的培养物 karenia mikimotoi。使用抑制减法杂交（SSH），然后使用454焦点测序和RT-QPCR方法，对ATP和+ P培养物进行差异基因表达。我们发现ATP支持与L1培养基相似的生长速率和细胞产率，并且观察到从ATP进入培养基中的浸渍释放，表明 k。 Mikimotoi </ i>细胞表达细胞外水解酶以水解ATP。然而，我们的SSH，QPCR和酶活性测定表明，5'-核苷酸酶（5NT），而不是AP，负责ATP水解。进一步的基因表达分析揭示了在利用ATP之后显着改变细胞间嘌呤代谢。我们的调查结果揭示了一种调节ATP利用率和P代谢的多面机机械。 mikimotoi </ i>，并且下划线AP活动不是DOP利用的独家指标。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" > 著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22868/'>《Environmental microbiology 》</a> <b style="margin: 0 2px;">|</b><span>2017年第11期</span><b style="margin: 0 2px;">|</b> <span>共13页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Luo Hao&option=202" target="_blank" rel="nofollow">Luo Hao;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lin Xin&option=202" target="_blank" rel="nofollow">Lin Xin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Ling&option=202" target="_blank" rel="nofollow">Li Ling;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lin Lingxiao&option=202" target="_blank" rel="nofollow">Lin Lingxiao;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Chao&option=202" target="_blank" rel="nofollow">Zhang Chao;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lin Senjie&option=202" target="_blank" rel="nofollow">Lin Senjie;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>State Key Laboratory of Marine Environmental Science Department of Marine Biological Sciences and Technology College of Ocean and Earth SciencesXiamen UniversityXiamen 361102 China;</p> <p>State Key Laboratory of Marine Environmental Science Department of Marine Biological Sciences and Technology College of Ocean and Earth SciencesXiamen UniversityXiamen 361102 China;</p> <p>State Key Laboratory of Marine Environmental Science Department of Marine Biological Sciences and Technology College of Ocean and Earth SciencesXiamen UniversityXiamen 361102 China;</p> <p>State Key Laboratory of Marine Environmental Science Department of Marine Biological Sciences and Technology College of Ocean and Earth SciencesXiamen UniversityXiamen 361102 China;</p> <p>Department of Biochemistry Province Key Laboratory of Biochip School of Basic Medical Science and Institute of Genetic EngineeringSouthern Medical UniversityGuangzhou China;</p> <p>State Key Laboratory of Marine Environmental Science Department of Marine Biological Sciences and Technology College of Ocean and Earth SciencesXiamen UniversityXiamen 361102 China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li> <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span> <a href="https://www.zhangqiaokeyan.com/clc/183.html" title="微生物学">微生物学 ;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> </p> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023229179.html">Transcriptomic and physiological analyses of the dinoflagellate <i xmlns="http://www.wiley.com/namespaces/wiley">Karenia mikimotoiKarenia mikimotoi reveal non‐alkaline phosphatase‐based 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Transcriptomic and physiological analyses of the dinoflagellate Karenia mikimotoiKarenia mikimotoi reveal non‐alkaline phosphatase‐based molecular machinery of ATP utilisation

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