Single cell observations show persister cells wake based on ribosome content

Kim Jun‐Seob; Yamasaki Ryota; Song Sooyeon; Zhang Weiwei; Wood Thomas K.

摘要

Summary > Since persister cells survive antibiotic treatments through dormancy and resuscitate to reconstitute infections, it is imperative to determine the rate at which these cells revive. Using two sets of Escherichia coli persister cells, those arising after antibiotic treatment at low levels and those generated at high levels by ceasing transcription via rifampicin pretreatment (shown to be bona fide persisters through eight sets of experiments), we used microscopy of single cells to determine that the resuscitation of dormant persisters is heterogeneous and includes cells that grow immediately. In all, five phenotypes were found during the observation of persister cells when fresh nutrients were added: (i) immediate division, (ii) immediate elongation followed by division, (iii) immediate elongation but no division, (iv) delayed elongation/division and (v) no growth. In addition, once cell division begins, the growth rate is that of exponential cells. Critically, the greater the ribosome content, the faster the persister cells resuscitate. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>摘要</ title> >自泄管细胞通过休眠抗生素治疗来活跃，重新刺激重组感染，必须确定这些细胞恢复的速率。使用两组大肠杆菌（Escherichia coli），抗生素治疗在低水平的抗生素治疗后产生的那些，通过利福平预处理通过释放转录产生高水平（通过八套实验显示BOA FIDE持久性），我们使用单细胞的显微镜检查，以确定休眠滞留的复苏是异质的，包括立即生长的细胞。总而言之，在加入新鲜营养物质时，在泄漏细胞期间发现了五种表型：（i）立即划分，（ii）立即伸长率，然后立即伸长，（iii）即时伸长率但没有划分，（iv）延迟伸长率/分裂（v）没有增长。此外，一旦细胞分裂开始，增长率就是指数细胞。危重性地，核糖体含量越大，渗漏细胞复苏的速度越快。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" > 著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22868/'>《Environmental microbiology 》</a> <b style="margin: 0 2px;">|</b><span>2018年第6期</span><b style="margin: 0 2px;">|</b> <span>共14页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kim Jun‐Seob&option=202" target="_blank" rel="nofollow">Kim Jun‐Seob;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yamasaki Ryota&option=202" target="_blank" rel="nofollow">Yamasaki Ryota;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Song Sooyeon&option=202" target="_blank" rel="nofollow">Song Sooyeon;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Weiwei&option=202" target="_blank" rel="nofollow">Zhang Weiwei;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wood Thomas K.&option=202" target="_blank" rel="nofollow">Wood Thomas K.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Chemical EngineeringPennsylvania State University University ParkPA 16802‐4400 USA;</p> <p>Department of Chemical EngineeringPennsylvania State University University ParkPA 16802‐4400 USA;</p> <p>Department of Chemical EngineeringPennsylvania State University University ParkPA 16802‐4400 USA;</p> <p>Department of Chemical EngineeringPennsylvania State University University ParkPA 16802‐4400 USA;</p> <p>Department of Chemical EngineeringPennsylvania State University University ParkPA 16802‐4400 USA;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li> <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span> <a href="https://www.zhangqiaokeyan.com/clc/183.html" title="微生物学">微生物学 ;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> </p> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023228660.html">Single cell observations show persister cells wake based on ribosome content</a> <b>[J]</b> . <span> <a 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