Quartz‐crystal Microbalance Measurements of CD19 Antibody Immobilization on Gold Surface and Capturing B Lymphoblast Cells: Effect of Surface Functionalization

Icoz Kutay; Soylu Mehmet Cagri; Canikara Zeynep; Unal Ekrem

摘要

Abstract > We have investigated different surface functionalization methods to immobilize CD19 antibody on gold surface to capture B lymphoblast cells associated with the acute lymphoblastic leukemia disease. Quartz Crystal Microbalance measurements were performed to analyze the binding kinetics of each layer and determine the optimum method, which results in higher cell capture rates. The random orientation of antibody and oriented antibody through protein G was investigated and protein G presence resulted in 15,2?Hz frequency shift for 10 4 ?cells/mL. The 3‐mercaptopropyltrimethoxysilane (MPS) and 11‐Mercaptoundecanoic acid (MUA) coatings of gold surface together with 4‐(N‐Maleimidomethyl)cyclohexane‐1‐carboxylic acid 3‐sulfo‐N‐hydroxysuccinimide ester sodium salt (Sulfo‐SMCC) and N‐Ethyl‐N’‐(3‐dimethylaminopropyl) carbodiimide hydrochloride (EDC)/N‐hydroxysulfosuccinimide (NHS) linker layers were tested on QCM for protein G and antibody binding. The results indicate that MUA, EDC/NHS, protein G, antibody CD19 is the optimum surface modification among the tested combinations. By using the optimum surface functionalization method, minimum 10 3 cell per mL was measured as 1.9?Hz frequency shift. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</标题> >我们已经研究了不同的表面官能化方法以固定CD19抗体对金表面上的CD19抗体以捕获B淋巴细胞细胞与急性淋巴细胞白血病疾病有关。进行石英晶微观测量以分析每层的结合动力学并确定最佳方法，从而导致更高的细胞捕获速率。研究了抗体和取向抗体通过蛋白质g的随机取向，并使蛋白G存在导致15,2〜Hz频率偏移10 4 </ sup>？细胞/ ml。金色表面的3-巯基丙基三甲氧基硅烷（MPS）和11-巯基癸酸（MUA）涂层与4-（N-马来酰亚胺甲基）环己烷-1-羧酸3-磺基-N-羟基琥珀酰亚胺钠盐（Sulfo-SMCC）以及在QCM上测试N-乙基-N' - （3-二甲基氨基丙基）碳二亚胺盐酸碳（EDC）/ N-羟基硫代琥珀酰亚胺（NHS）接头层，用于蛋白G和抗体结合。结果表明，MUA，EDC / NHS，蛋白G，抗体CD19是测试组合中的最佳表面改性。通过使用最佳表面官能化方法，测量每mL的最小10 3 </ sup>电池为1.9°频率偏移。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22546/'>《Electroanalysis》</a> <b style="margin: 0 2px;">|</b><span>2018年第5期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Icoz Kutay&option=202" target="_blank" rel="nofollow">Icoz Kutay;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Soylu Mehmet Cagri&option=202" target="_blank" rel="nofollow">Soylu Mehmet Cagri;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Canikara Zeynep&option=202" target="_blank" rel="nofollow">Canikara Zeynep;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Unal Ekrem&option=202" target="_blank" rel="nofollow">Unal Ekrem;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>BioMINDS (Bio Micro/Nano Devices and Sensors) Lab Department of Electrical and Electronics EngineeringAbdullah Gul University38080 Kayseri Turkey;</p> <p>Biomedical Engineering DepartmentErciyes University38030 Kayseri Turkey;</p> <p>Biomedical Engineering DepartmentErciyes University38030 Kayseri Turkey;</p> <p>Division of Pediatric Hematology Department of Pediatrics Faculty of MedicineErciyes University38030 Kayseri Turkey;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/4817.html" title="电化学、电解、磁化学">电化学、电解、磁化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=surface functionalization&option=203" rel="nofollow">surface functionalization;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=acute lymphoblastic leukemia&option=203" rel="nofollow">acute lymphoblastic leukemia;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=quartz crystal microbalance with dissipation&option=203" rel="nofollow">quartz crystal microbalance with dissipation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=CD19 immobilization&option=203" rel="nofollow">CD19 immobilization;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=B lymphoblast&option=203" rel="nofollow">B lymphoblast;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=protein G&option=203" rel="nofollow">protein G;</a> </p> <div class="translation"> 机译：表面官能化;急性淋巴细胞白血病;石英晶体微观耗散;CD19固定;B淋巴细胞;蛋白G.; </div> </li> </ul> </div> </div> <div class="literature cardcommon" id="literaturereference" style="display:none"> <div class="similarity "> <h3 class="all_title" id="enpatent111">引文网络</h3> <div class="referencetab clearfix"> <ul id="referencedaohang"> <li dataid="referenceul">参考文献</li> <li dataid="citationul">引证文献</li> <li dataid="commonreferenceul">共引文献</li> <li dataid="commoncitationul">同被引文献</li> <li dataid="tworeferenceul">二级参考文献</li> <li dataid="twocitationul">二级引证文献</li> </ul> </div> <div class="reference_details" id="referenceList"> <ul id="referenceul"></ul> <ul id="citationul"></ul> <ul id="commonreferenceul"></ul> <ul id="commoncitationul"></ul> <ul id="tworeferenceul"></ul> <ul id="twocitationul"></ul> </div> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023174391.html">Quartz‐crystal Microbalance Measurements of CD19 Antibody Immobilization on Gold Surface and Capturing B Lymphoblast Cells: Effect of Surface Functionalization</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Icoz Kutay&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Icoz Kutay,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Soylu Mehmet Cagri&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Soylu Mehmet Cagri,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Canikara Zeynep&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Canikara Zeynep,</a> <a href="/journal-foreign-22546/" target="_blank" rel="nofollow" class="tuijian_authcolor">Electroanalysis .</a> <span>2018</span><span>,第5期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：CD19抗体固定化对金表面和捕获B淋巴细胞细胞的晶晶微观测量：表面官能化的影响</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-foreign_detail_thesis/0204114840321.html">Antibody fragment immobilization on planar gold and gold nanoparticle modified quartz crystal microbalance with dissipation sensor surfaces for immunosensor applications</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=A. 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<a href="/conference-cn-34211/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 第八次全国生物化学与分子生物学学术会议 </a> <span> <span> . 1997</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020315836312.html">CD19抗体偶联药物对B淋巴细胞白血病抑制作用的研究</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=潘慧&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 潘慧</a> <span> . 2020</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120104976987.html">用于靶向结合淋巴细胞白血病细胞的抗CD19的工程抗体及其用途</a> <b>[P]</b> . <span> 中国专利： CN1775808A </span> <span> . 2006-05-24</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120112266244.html">可应用于肿瘤细胞捕获的小鼠抗细胞表面糖蛋白CD138的单克隆抗体</a> <b>[P]</b> . <span> 中国专利： CN112279917A </span> <span> . 2021-01-29</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130401234640.html">Method for modification of solid surfaces with negative surface charge, preferably surfaces of the quartz microbalance sensors, positively charged with nanoparticles of gold</a> <b>[P]</b> . <span> 外国专利：  PL234812B1 </span> <span> . 2020-04-30</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用负表面电荷修饰固态表面的方法，该表面最好是石英微量天平传感器的表面，并带有金纳米粒子带正电荷 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130406570270.html">Method for modification of solid surfaces with negative surface charge, preferably surfaces of the quartz microbalance sensors, positively charged with nanoparticles of gold</a> <b>[P]</b> . <span> 外国专利：  PL419878A1 </span> <span> . 2018-07-02</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用负表面电荷修饰固态表面的方法，该表面最好是石英微量天平传感器的表面，并带有金纳米粒子带正电荷 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130426794438.html">Detection device i.e. quartz microbalance, for detecting e.g. protein in fluid sample, has flat metallic thick- or thin layer arranged as electrode at channel wall, where ligands above electrode are immobilized on measuring surface</a> <b>[P]</b> . <span> 外国专利：  DE102008062620A1 </span> <span> . 2010-07-15</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：检测装置，例如石英微量天平，用于检测例如液体样品中的蛋白质，在通道壁上具有平坦的金属厚或薄层，作为电极排列，电极上方的配体固定在测量表面上 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> 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