Dual Amplified Electrochemical Immunosensor for Hepatitis B Virus Surface Antigen Detection Using Hemin/G‐Quadruplex Immobilized onto Fe        <sub >3</sub>3        O        <sub >4</sub>4        ‐AuNPs or (Hemin‐Amino‐rGO‐Au) Nanohybrid

Alizadeh Negar; Hallaj Rahman; Salimi Abdollah

摘要

Abstract > A sensitive electrochemical immunosensor for Hepatitis B virus surface antigen (HBsAg) detection was fabricated based on hemin/G‐quadruplex interlaced onto Fe <sub>3</sub> O <sub>4</sub> ‐AuNPs or hemin ‐amino‐reduced graphene oxide nanocomposite (H‐amino‐rGO‐Au). G‐quadruplex DNAzyme, which is composed of hemin and guanine‐rich nucleic acid, is an effective signal amplified tool for its outstanding peroxidase activity and Fe <sub>3</sub> O <sub>4</sub> ‐AuNPs or (H‐amino‐rGO‐Au) nanocomposites with quasi‐enzyme activity provide appropriate support for the immobilization of hemin/G‐quadruplex. The target protein was sandwiched between the primary antibody immobilized on the GO and secondary antibody immobilized on the Fe <sub>3</sub> O <sub>4</sub> ‐AuNPs or (H‐amino‐rGO‐Au) nanocomposites and glutaraldehyde was used as linking agent for the immobilization of primary antibody on the surface of GO. Both Fe <sub>3</sub> O <sub>4</sub> ‐AuNPs and H‐amino‐rGO‐Au nanocomposite and also hemin/G‐quadruplex can cooperate the electrocatalytic reduction of H <sub>2</sub> O <sub>2</sub> in the presence of methylene blue as mediator. The proposed immunosensor has a wide linear dynamic range of 0.1?pg/ml to 300?pg/ml with a detection limit of 60?fg/ml when Fe <sub>3</sub> O <sub>4</sub> ‐AuNPs was used for immobilization of hemin/G‐quadruplex, while the dynamic range and DL were 0. 1–1000?pg/mL and 10?fg/mL, respectively in the presence of H‐amino‐rGO‐ Au nanocomposite as platform for immobilizing of hemin/G‐quadruplex. The proposed immunosensor was also used for analysis of HBsAg in spiked human serum samples with satisfactory results. </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>摘要</标题> >乙型肝炎病毒表面抗原（HBsAg）的敏感电化学免疫传感器基于血红素制备/ g- quadruplex交织在Fe <sub> 3 </ sub> O <sub> 4 </ sub> -aUnps或血清氨基还原的氧化石氧化物纳米复合材料（H-amino-rgo-au）上。 G-Quadreplexyme由血葱和富含血红素核酸组成的，是其出色的过氧化物酶活性和Fe <sub> 3 </ sub> O <sub> 4 </ sub> -aUNP或者的有效信号放大工具（H-amino-rgo-Au）具有准酶活性的纳米复合材料提供了对血红素/ G-四丙烯的固定的适当载体。将靶蛋白夹在固定在固定在Fe <sub> 3 </ sub> O <sub> 4 </ sub> -aUnps或（H-amino-rgo-Au）纳米复合材料上的转储和次级抗体之间的初级抗体之间并且戊二醛用作将原发性抗体固定在去的表面上的连接剂。 Fe <sub> 3 </ sub> O <sub> 4 </ sub> -aUNP和H-amino-rgo-au纳米复合材料，并且血红素/ g-四边形可以配合H <sub> 2的电催化还原</.在亚甲基蓝色作为介体存在亚℃> O <sub> 2 </ sub>。所提出的免疫传感器的宽线性动态范围为0.1〜pg / ml至300·pg / ml，当Fe <sub> 3 </ sub> O <sub> 4 </ sub>时，检测极限为60〜fg / ml。 -aUnps用于固定血红素/ g-四边形，而动态范围和D1分别在H-amino-rgo-au纳米复合材料存在下分别为0.1-1000·pg / ml和10·fg / ml固定血红素/ G-quadruplex的平台。该提出的免疫传感器还用于分析尖刺人血清样品中的HBsAg，结果令人满意。 </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22546/'>《Electroanalysis》</a> <b style="margin: 0 2px;">|</b><span>2018年第3期</span><b style="margin: 0 2px;">|</b><span>共13页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Alizadeh Negar&option=202" target="_blank" rel="nofollow">Alizadeh Negar;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hallaj Rahman&option=202" target="_blank" rel="nofollow">Hallaj Rahman;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Salimi Abdollah&option=202" target="_blank" rel="nofollow">Salimi Abdollah;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of ChemistryUniversity of Kurdistan66177-15175 Sanandaj- Iran;</p> <p>Department of ChemistryUniversity of Kurdistan66177-15175 Sanandaj- Iran;</p> <p>Department of ChemistryUniversity of Kurdistan66177-15175 Sanandaj- Iran;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/4817.html" title="电化学、电解、磁化学">电化学、电解、磁化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fe 3 O 4 -AuNPs&option=203" rel="nofollow">Fe 3 O 4 -AuNPs;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=(Hemin-amino-rGO-Au) nanohybrid&option=203" rel="nofollow">(Hemin-amino-rGO-Au) nanohybrid;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hemin/G-quadruplex&option=203" rel="nofollow">Hemin/G-quadruplex;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hepatitis B virus surface antigen&option=203" rel="nofollow">Hepatitis B virus surface antigen;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=DNAzyme&option=203" rel="nofollow">DNAzyme;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nanozyme&option=203" rel="nofollow">Nanozyme;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Electrochemical immunosensor&option=203" rel="nofollow">Electrochemical immunosensor;</a> </p> <div class="translation"> 机译：Fe 3 O 4 -aUnps;（血葱 - 氨基-rgo-au）纳米冬次;血红素/ g-quadruplex;乙型肝炎病毒表面抗原;dnazyme;纳佐;电化学免疫传感器; 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Dual Amplified Electrochemical Immunosensor for Hepatitis B Virus Surface Antigen Detection Using Hemin/G‐Quadruplex Immobilized onto Fe 33 O 44 ‐AuNPs or (Hemin‐Amino‐rGO‐Au) Nanohybrid

摘要

Dual Amplified Electrochemical Immunosensor for Hepatitis B Virus Surface Antigen Detection Using Hemin/G‐Quadruplex Immobilized onto Fe ₃3 O ₄4 ‐AuNPs or (Hemin‐Amino‐rGO‐Au) Nanohybrid