Structural, morphological and magnetic properties of algae/CoFe2O4 and algae/Ag-Fe-O nanocomposites and their biomedical applications

摘要

Algae-assisted auto-combustion method was adopted to prepare Algae/CoFe2O4 and Algae/Ag-Fe-O nanoparticles (NPs). Structural properties of the samples were investigated using XRD (X-ray Diffraction) FITR (Fourier Transform Infra-red Spectroscopy), SEM (Scanning Electron Microscopy), and EDX (Energy Dispersive X-ray Analysis) and magnetic properties using VSM (Vibrating Sample Magnetometer). EDX spectra revealed the presence of expected stoichiometry in Algae/CoFe2O4 system but not in Algae/Ag-Fe-O. XRD patterns indicate the cubic phases of nanoparticles en-capsulated in the algae matrix. The sizes of the particles are found to be in the range of 15-21 nm. The room-temperature magnetic behaviour of the composites depends on the nature of dopant as Algae/CoFe2O4 NPs show ferromagnetic nature with significant coercivity whereas Algae/Ag-Fe-O NPs are super-paramagnetic. Invitro anti-proliferative effect of Co-NPs and Ag-NPs at different concentrations (10, 25, 50, 75, 100 mu g/ml) was evaluated against IMR 32 cell-line after 24 h incubation. The result of MIT assay affirmed that the cell-line deteriorates showing higher toxicity caused by Ag-NPs as compared to the results observed with the Co-NPs.