Hydrocarbon-Oxidizing Bacteria from Urban Lake Beloye (Moscow): Identification and Phylogenetic Analysis

摘要

Cultured hydrocarbon-oxidizing bacteria have been isolated for the first time from bacterioplankton in urban Lake Beloye (Moscow). The taxonomic positions of two bacterial strains (2012B and 2012C) isolated from this lake have been determined. Lipids of the strain 2012B comprise C-14:0-C(19:0)fatty acids, the most abundant of them being C-15:0(54%), C-16:0(17%), C-17:0(10%), and 10-methyl C-18:0(3.5%). Lipids of the strain 2012C comprise C-14:0-C(19:0)fatty acids, the most abundant of them being C1(5:0)(45%), C-16:0(32%), and C-17:0(9%). A phylogenetic analysis of strain 2012B is performed using the nucleotide sequences of the 16S rRNA (KP779654.1) andalkB(KR422620.1) genes and the strain is identified as a typical member of the genusRhodococcusspp. (Actinobacteria, Nocardiaceae). The combination of molecular identification and analysis of biochemical and physiological properties makes it possible to identify strain 2012B asRhodococcusqingshengii2012B. A phylogenetic analysis of strain 2012C is performed using the nucleotide sequences of 16S rRNA (MG966152) and shows the highest identity (99.57%) of strain 2012C withPseudomonas psychrotoleransandPseudomonas oryzihabitans