首页> 外文期刊>Industrial and organizational psychology >Population-level inferences from environmental DNA-Current status and future perspectives
【24h】

Population-level inferences from environmental DNA-Current status and future perspectives

机译:来自环境DNA-Current状态和未来观点的人口水平推论

获取原文
获取原文并翻译 | 示例
获取外文期刊封面目录资料

摘要

Environmental DNA (eDNA) extracted from water samples has recently shown potential as a valuable source of population genetic information for aquatic macroorganisms. This approach offers several potential advantages compared with conventional tissue-based methods, including the fact that eDNA sampling is noninvasive and generally more cost-efficient. Currently, eDNA approaches have been limited to single-marker studies of mitochondrial DNA (mtDNA), and the relationship between eDNA haplotype composition and true haplotype composition still needs to be thoroughly verified. This will require testing of bioinformatic and statistical software to correct for erroneous sequences, as well as biases and random variation in relative sequence abundances. However, eDNA-based population genetic methods have far-reaching potential for both basic and applied research. In this paper, we present a brief overview of the achievements of eDNA-based population genetics to date, and outline the prospects for future developments in the field, including the estimation of nuclear DNA (nuDNA) variation and epigenetic information. We discuss the challenges associated with eDNA samples as opposed to those of individual tissue samples and assess whether eDNA might offer additional types of information unobtainable with tissue samples. Lastly, we provide recommendations for determining whether an eDNA approach would be a useful and suitable choice in different research settings. We limit our discussion largely to contemporary aquatic systems, but the advantages, challenges, and perspectives can to a large degree be generalized to eDNA studies with a different spatial and temporal focus.
机译:从水样中提取的环境DNA(EDNA)最近显示出潜在的水生宏大群体遗传信息源。与传统的基于组织的方法相比,这种方法提供了多种潜在的优点,包括EDNA采样是非侵入性的并且通常更具成本效益的事实。目前,EDNA方法仅限于线粒体DNA(MTDNA)的单标记研究,eDNA单倍型组成和真正的单倍型组合物之间的关系仍然需要彻底验证。这将需要测试生物信息和统计软件,以纠正错误的序列,以及相对序列丰富的偏差和随机变化。然而,基于EDNA的人口遗传方法对基础和应用研究具有深远的潜力。在本文中,我们迄今为止介绍了基于EDNA的人口遗传学的成就,并概述了该领域未来发展的前景,包括核DNA(NUDNA)变异和表观遗传信息的估算。我们讨论与EDNA样品相关的挑战,而不是单个组织样本的挑战,并评估EDNA是否可以提供与组织样品无法获得的额外类型的信息。最后,我们提供了确定EDNA方法是否在不同的研究环境中是一个有用和合适的选择的建议。我们主要将我们的讨论限制在很大程度上,对当代水生系统,但优势,挑战和观点可以在很大程度上推广到EDNA研究,具有不同的空间和时间焦点。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号