Improving diosgenin production and its biosynthesis in Trigonella foenum-graecum L. hairy root cultures

摘要

Induction of hairy roots stemmed from Agrobacteriwn rhizogenes transformation, is a key step to reach mass production of secondary metabolites in medicinal plants. Accordingly, this study is aimed to investigate potential impact of different A. rhizogenes strains, including ATCC15834, R1000, A4 and C58, on diosgenin biosynthesis in high- "Boshruyeh" (23.8 mg/gDW) and low "Hamadan" (6.4 mg/gDW) diosgenin-producing genotypes of fenugreek. Interestingly, comparing the metabolite extracted from both leaves and hairy roots of these genotypes suggested that hairy roots are promising platform to produce scalable amount of diosgenin, producing 143.96 mg/gDW in high diosgenin producing hairy roots (A4-mediated hairy roots induction in "Boshruyeh" (BA4)), while 23.8 mg/gDW in high diosgenin producing genotype. Transcript abundance of 10 genes involved in diosgenin biosynthesis pathway as well as three rate-limiting genes; i.e 1-deoxy-D-xylulose-5-phosphate synthase (DXS), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) and 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, functioning in plastid 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway were measured. Comparison of the transcription and metabolite analysis revealed that the more expression of Delta(24)-reductase gene, the higher accumulation of diosgenin in the hairy roots. In high diosge nin producing hairy roots, the expression of Delta(24)-reductase gene were significantly up-regulated (8-folds). Moreover, integration of metabolite and transcript results revealed that A4 has a strong capacity in boosting diosgenin accumulation in the both genotypes. These results may lead to the next step in production of diosgenin in scalable and commercial levels.