Essential oil of Eucalyptus camaldulensis Dehn potentiates beta-lactam activity against Staphylococcus aureus and Escherichia coli resistant strains

摘要

The emergence of multi-drug resistant bacteria is a serious worldwide health problem which has promoted the search for strategies aimed at tackling these pathogens. Among such strategies, the development of new antimicrobial drugs and the discovery of optimizing agents related to the existing ones stand out. This study evaluated the chemical composition of the Eucalyptus cumaldulensis Dehn essential oil (EOEc) and its potential as an antimicrobial agent, as well as its potential a resistance modifier of beta-lactamase-producing and Methicillin-resistant Staphylococcus aureus (MRSA) strains to beta-lactam antibiotics. The EOEc was obtained through hydro distillation and its chemical profile through GC/MS presenting a total of five compounds with 1,8 cineole (76.93%), beta-pinene (11.49%) and alpha-pinene (7.15%) as its major constituents. The microdilution test was carried out in order to determine the Minimal Inhibitory Concentration (MIC) and the bacterial resistance modulation of the EOEc. The oil presented antimicrobial activity over the S. aureus 29 and MRSA strains (MIC = 1000 mu g mL(-1)). Mixtures of the essential oil and antibiotics resulted in synergistic effects, mainly with amoxicillin and ampicillin for S. aureus 29 and MRSA, and cephalexin and cefuroxime for E. coil. The EOEc presented antimicrobial and beta-lactams optimizer potential against resistant strains, thus constituting a new therapeutic resource against resistant bacterial strains.