Spectral Intensity Bioaerosol Sensor (SIBS): an instrument for spectrally resolved fluorescence detection of single particles in real time

摘要

Primary biological aerosol particles (PBAPs) in the atmosphere are highly relevant for the Earth system, climate, and public health. The analysis of PBAPs, however, remains challenging due to their high diversity and large spatiotemporal variability. For real-time PBAP analysis, light-induced fluorescence (LIF) instruments have been developed and widely used in laboratory and ambient studies. The interpretation of fluorescence data from these instruments, however, is often limited by a lack of spectroscopic information. This study introduces an instrument - the Spectral Intensity Bioaerosol Sensor (SIBS; Droplet Measurement Technologies (DMT), Longmont, CO, USA) - that resolves fluorescence spectra for single particles and thus promises to expand the scope of fluorescent PBAP quantification and classification.