POPULATION STRUCTURE OF RACES OF Colletotrichum lindemuthianum

摘要

Anthracnose is a disease caused by the fungus Colletotrichum lindemuthianum (Sacc and Magnus) Briosi and Cavara. Anthracnose can lead to a yield reduction of up to 100% in common bean {Phaseolus vulgaris L.), when the conditions are favorable for thepathogen (Sileshi et al., 2014). C. lindemuthianum is characterized by a wide pathogenic variability with approximately 256 races identified worldwide (Nunes et al., 2013; Padder et al., 2017). There are different strategies to estimate this variability,such as amplification of the ribosomal DNA (rDNA) ITS (Internal Transcribed Spacer) regions via PCR (Polymerase Chain Reaction). ITS markers are used to amplify the ITS1 and ITS2 regions, which are repeated several times in the genome (Fungaro, 2000). For that reason, sequencing of these regions is an efficient way to estimate genetic variability in fungi. This study had as objective to evaluate the occurrence of the genetic differentiation of races of C. lindemuthianum from Brazil through the sequencing of the ITS regions.