IDENTIFICATION OF THE LEVULINATE DEHYDRATASE (ALAD) GENE POLYMORPHISM AND WHOLE BLOOD HEMOGLOBIN IN THE STUDENTS OF ELEMENTARY SCHOOL IN KALIDERES,JAKARTA, INDONESIA

摘要

Lead (Pb) is a toxic pollutant and no organism can decrease the natural concentration. In Kalideres, there is a bus station and industries that are adjacent to settlements, exposing children around to Pb. One of the characteristics of Pb particulatesis that they are found in the bottom layer of air, ± 1 meter above ground, equivalent to the height of childrenaged 6to 9 years old (grade 1 to 2 elementary school students) whose height range between 100-140 cm, increasing the risk of the childrenbeingexposed to Pb.Theaim of this study was to identify the ALAD gene polymorphism, to discover the frequencyof the ALAD gene polymorphism genotype, andto discover the relationship between the ALAD gene polymorphismgenotype and the hemoglobin concentration.The methodused in this study was a comparative-descriptive method with laboratory test techniques using a digital hemoglobinometer, PCR and PCR-ARMS (Amplification Refractory Mutation System), and the completion of questionnaires by the parentsfor respondent behavioral characteristicsandroutines. The samples were 60 students who were 51.7% were ALAD wildtypes, 48.3% heterozygoticgenotypes, and nomutants were found in the populationof elementary schoolchildren in Kalideres, Jakarta, and there was no connection between the ALAD gene polymorphismgenotype and the hemoglobin concentration category (anemicandnon-anemic). The results of this research also predict profile level of lead environmental present as well.