Simple Method for High Sensitivity Chemilumi-nescence ELISA Using Conventional Laboratory Equipment

摘要

Enzyme-linked immunosorbent assays (ELISAs) have a unique importance in numerous different analytical procedures due to their specificity and sensitivity. Moreover, the use of the 96-well format allows the quantification of high numbers of samples inparallel. Most .commonly, the binding of antibodies to antigens in an ELISA is detected by the cleavage of a chro-mogenic substrate by an enzyme conjugate, for instance alkaline phos-phatase or horseradish peroxidase (HRP) coupled to either an antibody or to streptavidin. Thus it is usually a col- ored reaction product that is quantified by measuring the absorbance. Assays with higher sensitivities can be set up based on the principle of chemilumi-nescence (2-5), but equipment for chemiluminescence measurements in a 96-well format are more expensive than the usual ELISA plate readers and therefore not available in many laboratories.