Beyond the Cuvette: Redox Indicators in Biological Experiments

摘要

Redox signaling is involved in numerous physiological and pathological processes (cell cycle, gene transcription, calcium signaling, stress response, ischemia-reperfusion injury, etc.). However, its exact role in cell biology and physiology remains poorly understood, mostly due to the technical challenges that the experimenter faces while trying to detect reactive oxygen species (ROS) or redox species with adequate specificity, spatial, and temporal accuracy. Recently, tremendous efforts have been put into the development of techniques for redox detection. This Forum focuses on ex and in vivo live-imaging of ROS and redox species using fluorescent dyes.