Molecular Biology Techniques Q&A

摘要

This month's question from the Molecular Biology Forums (online at molecularbiology.forums.biotechniques.com) comes from the "Real-Time qPCR/qRT-PCR Methods" section. Entries have been edited for concision and clarity. Mentions of specific products and manufacturers have been retained from the original posts, but do not represent endorsements by, or the opinions of, BioTechniques. Q: I've always used SYBR Green or HYBProbe master mix kits for my qPCR reactions. But for my current project, I need to sequence the products following qPCR. My advisor says that a high fidelity enzyme should be used here since we will be sequencing the products, which means I can't use the master mixes.