Construction of a novel ratiometric near-infrared fluorescent probe for SO2 derivatives and its application for biological imaging

摘要

Sulfur dioxide (SO2) is one of the most important reactive sulfur species widely found in living organisms. SO2 is recognized as an important regulatory factor that can modulate the cardiovascular system, with functions such as relaxing blood vessels, lowering blood pressure and acting as an anti-oxidation. Compared to fluorescence turn-on type probes, a ratiometric fluorescent probe can avoid the background fluorescence of the probe and the interference from other environmental factors. Herein, a new ratiometric-type fluorescent probe with emission in the near infrared area was developed. Upon interaction with SO2, the emission at 750 nm decreased and a new fluorescent peak was observed in the visible region simultaneously. The novel probe showed excellent selectivity toward SO2 among other species. In addition, the new probe exhibited little toxicity to HeLa cells and was used for imaging SO2 in solution and the cell environment successfully with a fluorescence change in both the green and red channels.