Two highly sensitive and selective coumarin-based fluorometric probes for the detection of ClO- and cell imaging

摘要

Two coumarin-based compounds, 2-acetyl-3H-benzo[f] chromen-3-one (BCO) and (1E)-1-(1-(3-oxo-3Hbenzo[ f]-chromen-2-yl) ethylidene) thiosemicarbazide (BETC), were designed as excellent fluorescent probes for the detection of ClO in CH3OH : H2O (v/v = 1 : 1). The oxidizing reaction between the BCO & BETC probes and ClO induced an obvious fluorescence enhancement together with a color transformation from colorless to blue. The probes showed preferential selectivity towards ClO over various anions and metal ions. The detection limits of BCO and BETC were 154 nM and 32 nM, respectively. Interestingly, the tested reactive species, which include cOH, 1O2, and H2O2 do not interfere with the tests of ClO . Furthermore, cell imaging using HepG2 cells revealed that BCO & BETC could be applied as effective fluorescent probes for the detection of ClO in HepG2 cells.