Electrochemical study of the interaction mechanism of proflavine (PF) with DNA using carbon paste (CPE) and hanging mercury drop (HMDE) electrode

摘要

Proflavine binds with DNA in a complicated manner. This work involves the electrochemical study of this interaction using differential pulse voltammetry at a carbon paste electrode (CPE) and alternating current voltammetry at a hanging mercury drop electrode (HMDE). At the CPE the peak current intensity at 1.0V (corresponding to the oxidation of the guanine residues) decreased by increasing the concentration of proflavine. At the HMDE, a decrease in the current intensity of the DNA peak at-1.2V (corresponding to segmental desorption) was also observed by increasing the concentration of proflavine. These results confirmed, electrochemically, that proflavine intercalates within the DNA double helix and changes its conformation.