Antioxidant, Antidiabetic and Anticancer Activities of L-Phenylalanine and L-Tyrosine Ester Surfactants: In Vitro and In Silico Studies of their Interactions with Macromolecules as Plausible Mode of Action for their Biological Properties | Bentham Science

摘要

Background: Aromatic amino acid-based surfactants have been found to have interestingbiological properties such as antibacterial and hemolytic activities. Recently, we have reported the antibacterialactivity of a range of ester hydrochloride surfactants derived from L-Phenylalanine and LTyrosine.This study aims at assessing the antioxidant, α-glycosidase inhibitory and cytotoxic activitiesof a series of L-Phenylalanine and L-Tyrosine ester hydrochlorides. Molecular docking and BSA bindingstudies were also carried out in order to investigate their potential therapeutic targets.Methods: L-Phenylalanine and L-Tyrosine surfactants were tested as potential lipophilic antioxidantsusing the DPPH and ABTS assays. These surfactants were also tested for their α-glycosidase inhibitoryactivity using 4-nitrophenyl α -D-glucopyranoside (pNPG) as substrate. Their cytotoxicity effects werescreened using HeLa and KB cell lines. Glide version 5.7 as implemented in Schr?dinger suite 2013-1,was used for performing docking studies of L-Phenylalanine and L-Tyrosine dodecyl esters. The interactionof the ester hydrochlorides of L-Phenylalanine and L-Tyrosine with bovine serum albumin (BSA)was investigated using fluorometric titration.Results: The presence of the phenolic moiety in L-Tyrosine-based surfactants was found to enhance theantioxidant and α-glucosidase inhibitory activities compared to the L-Phenylalanine derivatives. The α-glucosidase and anticancer activities of the phenylalanine surfactants were found to increase with chainlength up to C12 above which the activities exhibited a downward trend. In the case of the tyrosine series,an increase in chain length from C8 to C14 was found to decrease the α-glucosidase inhibitoryactivity and increase the anticancer activity of the surfactants. Binding studies with bovine serum albuminshowed that the tyrosine surfactants displayed greater affinity for the serum albumin, owing to thepresence of the phenolic group which altered the orientation of the surfactant molecule within the hydrophobiccore of BSA.Conclusion: L-Tyrosine esters having a phenolic moiety were found to possess enhanced biologicalactivity in terms of both the antioxidant and antidiabetic activities as well as also bind more strongly toBovine serum albumin. Molecular docking studies of the phenylalanine and tyrosine surfactants of similarchain length with target proteins showed direct correlation with their anticancer and antidiabeticactivity. Therefore, the findings show that these aromatic based surfactants derived from L-Tyrosine canact as promising antioxidant, antidiabetic and anticancer agents, and they can also be efficiently transportedand eliminated in the body, making them useful candidates for drug designs.