Smooth Muscle Alpha Actin Immunoexpression (-Sma) and CD-117 Antibody (C-Kit) in Capsules Formed by Polyurethane Foam-Coated Silicone Implants and with Textured Surface: A Study on Rats

摘要

BackgroundOne of the undesirable complications that might occur after breast augmentation with silicone implants is capsular contracture. In its etiology, the relations between mast cells and myofibroblasts play an important role in collagen synthesis. Mast cells are able to activate fibroblasts into myofibroblasts, through paracrine secretions, inducing collagen production. The objectives of this study were to analyze the myofibroblast concentration through the -SMA immunomarker and evaluate the intensity of mast cell expression against the C-Kit immunomarker.Material and MethodSixty-four Wistar rats were used, divided into two groups (polyurethane foam and textured surface) with 32 animals in each. The animals received silicone implants on the back, below the panniculus carnosus, and after the determined period, they were killed and the capsules formed around the implants were studied. The capsules were analyzed employing the immunohistochemical technique, with the -SMA and C-Kit immunomarkers in subgroups of 30, 50, 70 and 90days.ResultsThe myofibroblast concentration was higher in the polyurethane group when compared to the textured group (30days p=0.105; 50days p=0.247; 70days p=0.014 and 90days p=0.536). The intensity of mast cell expression was more pronounced in the polyurethane group when compared to the textured group (30days p=0.798; 50days p=0.537; 70days p=0.094 and 90days p=0.536).ConclusionsPolyurethane-coated implants induced higher concentrations of myofibroblasts and higher expression of mast cells, when compared to the textured surface implants.No Level AssignedThis journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.