Development of FibroChip, a functional DNA microarray as a targeted metatranscriptomic tool to monitor carbohydrate-active enzyme gene expression by rumen microbiota

摘要

Biotic and abiotic factors influence the efficiency of fibre degradation in the rumen of ruminants, which can ultimately affect animal productivity and health (Chaucheyras-Durand et al., 2012). Up to now, knowledge gaps remain on how the activity of rumen fibrolytic microorganisms is modulated at the transcriptomic level. Therefore, a functional DNA microarray targeting genes encoding key enzymes involved in cellulose and hemicellulose degradation by rumen microbiota was designed. The FibroChip was used to analyse the transcriptome of the rumen cellulolytic bacterium Fibrobacter succinogenes and to characterise the expression of the carbohydrate active enzyme (CAZyme) gene repertoire of the rumen microbiota of a dairy cow fed a mixed diet based on grass silage.