Improved Strategy for Large-Scale DNA Sequencing Using DNasel Cleavage for Generating Random Subclones

摘要

Large-scale DNA sequencing projects require the use of specific and reliable strategies. Here, we describe an improved strategy using DNasel cleavage and sequencing from both ends of the plasmid inserts. This strategy yields better results than thoseobtained using partial digestion with restriction enzymes and cloning in single-stranded vectors.