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High‐Definition X‐Ray Imaging of Small Gecko Skin Surface Protuberances for Digitization and 3D Printing

机译：小壁虎皮肤表面突起的高清X射线成像进行数字化和3D打印

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Abstract > A detailed analysis of zoological and botanical engineering structures reveals remarkable ideas for developing new healthcare technologies. One eclectic example is the surface microprotrusions of Gecko lizard skins, featuring nanoscale tips that engage in bacterial repulsion and killing. However, building biomimetic synthetic nanostructures in long range for technological use is hampered by the lack of concentrated digital information for 3D nano‐fabrication. For instance, standard micro‐computed tomography ( μ CT), confocal laser scanning microscopy (CLSM) and atomic force microscopy (AFM) imaging failed to replicate gecko spinules at the nanoscale. The authors now show that the latest generation of high‐power X‐ray microscopy (Zeiss X‐Radia 810) builds high contrast images of the gecko skin surface, and following 3D digital conversion the individual spinules, in the large array, are perfectly delineated at 150 nm and clearly featured the 50 nm nanotips crucial for bacterial cell rupturing. Consequently, the authors generate stenographic (STL.) digital file formats loaded with the instructions for 3D printing and the design blueprints for soft lithography replication. A further reconstruction of the digital data is undertaken into a 3D solid object using MeshLabs, yielding biologically realistic, virtual‐world spinule arrays. In this form, the authors are able to edit the spinules for optimal killing performance against bacterial cells. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 对动物植物和植物工程结构的详细分析揭示了开发新的医疗技术的显着思想。一个折衷的例子是壁虎蜥蜴皮的表面微介林，具有纳米级尖端，可从事细菌排斥和杀伤。然而，通过缺乏3D纳米制造的浓缩数字信息，在长距离技术用途中建立仿生合成纳米结构。例如，标准微计算机断层扫描（ μ</ i> CT），共聚焦激光扫描显微镜（CLSM）和原子力显微镜（AFM）成像未能在纳米级上复制壁虎纺丝。作者现在表明，最新一代的高功率X射线显微镜（Zeiss X-Radia 810）构建了壁虎皮肤表面的高对比度图像，并且在3D数字转换之后，在大型阵列中，各个梭菌均完全描绘在150nm处，明显地精细介绍了50 nm纳米，对细菌细胞破裂至关重要。因此，作者生成了装载的速记（STL。）数字文件格式，用于3D打印指令和软光刻复制的设计蓝图。使用Meshlabs进行了进一步重建数字数据，以产生生物学逼真的虚拟世界旋阵阵列。在这种形式中，作者能够编辑纺丝梭，以获得对细菌细胞的最佳杀死性能。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-18063/'>《Advanced materials interfaces》</a> <b style="margin: 0 2px;">|</b><span>2018年第13期</span><b style="margin: 0 2px;">|</b><span>共12页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Green David W.&option=202" target="_blank" rel="nofollow">Green David W.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kelly Stephen T.&option=202" target="_blank" rel="nofollow">Kelly Stephen T.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lee Kenneth Ka‐Ho&option=202" target="_blank" rel="nofollow">Lee Kenneth Ka‐Ho;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Watson Gregory S.&option=202" target="_blank" rel="nofollow">Watson Gregory S.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Watson Jolanta A.&option=202" target="_blank" rel="nofollow">Watson Jolanta A.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jung Han Sung&option=202" target="_blank" rel="nofollow">Jung Han Sung;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Division in Anatomy and Developmental BiologyYonsei University College of DentistrySeoul 03722 Korea;</p> <p>Carl Zeiss Microscopy LLCZEISS Group4385 Hopyard Rd #100 Pleasanton CA 94588 USA;</p> <p>Key laboratory for Regenerative MedicineThe Chinese University of Hong KongShatin Hong Kong SAR;</p> <p>School of Science &</p> <p>EngineeringUniversity of the Sunshine CoastHervey Bay QLD 4655 Australia;</p> <p>School of Science &</p> <p>EngineeringUniversity of the Sunshine CoastHervey Bay QLD 4655 Australia;</p> <p>Division in Anatomy and Developmental BiologyYonsei University College of DentistrySeoul 03722 Korea;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/6960.html" title="特种结构材料">特种结构材料;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=3D bioimaging&option=203" rel="nofollow">3D bioimaging;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=CLSM&option=203" rel="nofollow">CLSM;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=gecko skin&option=203" rel="nofollow">gecko skin;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=nanoscale imaging&option=203" rel="nofollow">nanoscale imaging;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=X‐ray microscopy&option=203" rel="nofollow">X‐ray microscopy;</a> </p> <div class="translation"> 机译：3D BioImaging;CLSM;壁虎皮;纳米级成像;X射线显微镜; 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