The Thermodynamic and pH metric Studies on the Binding of Hg+2 and Mo+2 with RNA by Polarographic and Spectrometric Techniques

摘要

Background: Metalloproteins and metal-protein complexes play key roles in allorganisms. For example, certain metalloproteins are involved in metal homeostasis anddetoxification, or oxidative stress protection; whereas, metals serve as essential cofactorsin a large number of metal-protein complexes.Objective: Present work attempts to critically review methods for assessing the structure,characterization of the metal binding sites, with RNA involved in the various process of aliving being are discussed.Method: The binding of Hg(II) and Mo (II) ions have been studied with RNA at differentpH values and temperatures by the polarographic and spectrometric techniques. It isobserved that binding data depend on pH and temperature.Results: The intrinsic association constants (k) and the number of binding sites (n) werecalculated from Scatchard plots. The free energies of aggregation, ΔG associated with thebinding interaction of the Hg (II) and Mo(II) and RNA were calculated. The negativevalues of the ΔG confirm the feasibility of interaction between the metals and RNA.Conclusion: All the observations recorded in this paper indicate that the Hg (II) and Mo(II) ions have a good affinity of binding with RNA and the number of binding sites isdependent on various physical and chemical factors. Both ions when bind with the RNAinduce metabolic function at tissue level in fishes in the aquatic environment.