iTRAQ-based proteomic analysis of DMH-induced colorectal cancer in mice reveals the expressions of beta-catenin, decorin, septin-7, and S100A10 expression in 53 cases of human hereditary polyposis colorectal cancer

摘要

PurposeThe aim of this study is to explore the roles of -catenin, decorin, septin-7, and S100A10 expression in colorectal cancer development.MethodsTwenty-five BALB/c mice were divided into five groups; four groups were administrated N,N-dimethylhydrazine for 0, 10, 15, and 20weeks, and one group was administrated normal saline for 20weeks. The colons were collected for histopathological analysis. Protein samples prepared from the frozen colon tissues of mice treated with N,N-dimethylhydrazine for the different time points were evaluated using the isobaric tags for relative and absolute quantification (iTRAQ) labeling technique coupled with the 2D liquid chromatography-tandem mass spectrometry analysis. Based on the proteomic analysis results, immunohistochemical staining of -catenin, decorin, septin-7, and S100A10 was performed in paraffin-embedded mice colorectal tissue, and 53 cases of human hereditary polyposis colorectal cancer samples.ResultsColorectal cancer was observed in mice treated with N,N-dimethylhydrazine for 20weeks, and adenomas were observed in mice subjected to the 10-, and 15-week treatments. Seventy-two differentially expressed proteins were involved in the development of cancer as per the iTRAQ and spectrometry analysis. In normal epithelium, adenoma, and cancer from human hereditary polyposis colorectal cancer, S100A10 expression (c2=100.989, P=0.000) was highest in cancer, whereas decorin (c2=12.852, P=0.002) and septin-7 (c2=66.519, P=0.002) expressions were highest in the normal epithelium, which was confirmed via immunohistochemical staining.ConclusionsThe subcellular localization of -catenin and decorin, septin-7, and S100A10 expressions are associated with the development of colorectal cancer in mice after N,N-dimethylhydrazine treatment and in human hereditary polyposis colorectal cancers.