Association between promoter polymorphism (?108CT) of paraoxonase1 gene and it ' s paraoxonase activity in patients with Type2 diabetes in northern Iran

摘要

Abstract Type 2 diabetes mellitus (T2DM) is one of the diseases which depend on the obesity associated with insulin resistance. Various factors, such as a reduction in the activity of paraoxonase-1 enzyme (PON1), could affect T2DM. PON1 is a multifunctional enzyme with paraoxonase and arylesterase activities. The Activity of PON1 is influenced by various SNPs. The aim of presence study is to investigate the association between promoter polymorphism (?108C>T) of PON1 gene and its paraoxonase activity in patients suffering from Type 2 diabetes in Golestan Province, north of Iran. To this end, genomic DNA was extracted from whole blood then genotyping was carried out using PCR-RFLP and enzymatic activity of paraoxonase was measured in the serum of 90 healthy subjects and 90 diabetic patients. Data was analyzed using SPSS version 16. The relationship between the level of paraoxonase activity with polymorphisms of CC, CT, and TT was statistically significant in patients with T2DM. There was a significant association between polymorphism C-108>T of PON1 and type2 diabetes mellitus, with 24.4% of control group subjects and 15.5% of patients having CC genotype; p p p Highlights ? Paraoxonase activity of PON-1 decreases in patients with T2DM. ? There are three genotypes including CC, CT and TT in both control and patients with T2DM CT genotype was the most frequent genetype in both control and patients with T2DM TT is the most frequent genotype in patient with T2DM. ? TT genotype may reduce paraoxonase1enzymatic activity in patients with Type2 diabetes.