Rutaecarpine attenuates osteoclastogenesis by impairing macrophage colony stimulating factor and receptor activator of nuclear factor κ‐B ligand‐stimulated signalling pathways

摘要

Summary Rutaecarpine is a major alkaloid isolated from Evodia rutaecarpa . Here, we investigated the effects of rutaecarpine on osteoclast differentiation induced by macrophage colony stimulating factor (M‐ CSF ) and receptor activator of nuclear factor κ‐B ligand ( RANKL ) in bone marrow‐derived macrophages ( BMM s). Treatment with rutaecarpine significantly inhibited osteoclastogenesis and prevented bone resorption of BMM ‐derived osteoclasts. Mechanistically, rutaecarpine decreased the protein level of nuclear factor of activated T cells cytoplasmic‐1 ( NFAT c1) and the phosphorylation of other signalling pathways during the osteoclast differentiation. Thus, rutaecarpine may be useful as a therapeutic agent for the treatment of bone diseases.