IDENTIFICATION OF BACTERIAL CANKER CAUSED BY PSEUDOMONAS SYRINGAE PV. SYRINGAEON SWEET CHERRY TREES IN TEKIRDAG

摘要

Sweet cherry (Prunus avium L.) is one of the most important fruit trees grown in Tekirdag. Bacterial canker, which reduces the yield and quality of sweet cherry fruit and causes death of trees, was investigated. For this purpose, a survey study was conducted in 2012-2013, and 129 plant tissue samples were collected from symptomatic trees. Forty-one isolates from naturally infected plants were isolated and subjected to biochemical and pathogenicity tests. All strains were fluorescent on KB. On KB medium, most of the isolates produced slowly growing small, greyish-white colonies as reported previously (Lelliott and Stead, 1987). The reaction of P. syringae pv. syringae strains was positive for levan production; hypersensitive reaction on tobacco, gelatin hydrolysis, aesculine hydrolysis and negative for oxidase reaction, pectinolytic activity, arginine dehydrolase, tyrosine, tartaric acid. PCR studies were performed in a thermal cycler using primers, Bl (5'-CTTTCCGTGGTCTTGATGAGG- 3')-B2 (5'-TCGATTTT-GCCGTGATGAGTC- 3') specific for P. syringae pv. syringae for amplification of 752 bp of the syrB gene. In total, 41 bacterial strains of Pseudomonas syringae were determined by using classical and molecular identification tests, 18 strain were identifiedas Pseudomonas syringae pv. syringae.