Does norepinephrine influence pain behavior mediated by dorsal root ganglia?: a pilot study.

摘要

BACKGROUND: Postganglionic neurons in the sympathetic nervous system reportedly are involved in lumbar radicular pain and release norepinephrine (NE), a neurotransmitter. Increased numbers of sympathetic nerve fibers have been found in dorsal root ganglion (DRG) neurons in a root constriction model. Whether this is a reasonable model for pain, however, is unclear QUESTIONS/PURPOSES: We asked whether: (1) painful behaviors occurred in the root constriction model; (2) NE enhanced the excitability of DRG neurons in the root constriction model; and (3) which adrenoceptors were related to the mediation of the NE effects. METHODS: The L5 root was sutured proximal to the DRG as the root constriction model. Behavioral tests were performed until 28 days after surgery. At 10 to 14 days after the root constriction, DRG neurons were quickly excised and digested with collagenase for electrophysiologic studies. Action potentials were recorded from single DRG neurons using a whole-cell patch clamp technique. NE (10 mumol/L) was directly applied to the DRG neurons. The adrenergic sensitivity was examined in combination with antagonists. RESULTS: The rats with root constriction exhibited painful behavior. NE increased the excitability of DRG neurons in the root constriction model. The effects of NE were inhibited by pretreatment with an alpha-antagonist and alpha(2)-antagonist but not an alpha(1)-antagonist. CONCLUSIONS: Our observations suggest NE plays an important role in generating lumbar radicular pain mainly via alpha(2)-adrenoceptors. CLINICAL RELEVANCE: An alpha(2)-antagonist may be an appropriate agent for trials to treat lumbar radicular pain.