H2 b /J mouse model

摘要

Abstract Animal models that recapitulate human disease are crucial for the study of Sj?gren's Syndrome (SS). While several SS mouse models exist, there are few primary SS (pSS) models that mimic systemic disease manifestations seen in humans. Similar to pSS patients, NOD.B10Sn- H2 b /J (NOD.B10) mice develop exocrine gland disease and anti-nuclear autoantibodies. However, the disease kinetics and spectrum of extra-glandular disease remain poorly characterized in this model. Our objective was to characterize local and systemic SS manifestations in depth in NOD.B10 female mice at early and late disease time points. To this end, sera, exocrine tissue, lung, and kidney were analyzed. NOD.B10 mice have robust lymphocytic infiltration of salivary and lacrimal tissue. In addition, they exhibit significant renal and pulmonary inflammation. We identified numerous autoantibodies, including those directed against salivary proteins. In conclusion, the NOD.B10 model recapitulates both local and systemic pSS disease and represents an excellent model for translational studies. Highlights ? NOD.B10 mice represent an excellent model for primary Sj?gren's syndrome (pSS). ? NOD.B10 females exhibit sialadenitis and dacryoadenitis and lose salivary flow. ? NOD.B10 mice also show extra-glandular manifestations of pSS. ? Similar to some pSS patients, NOD.B10 females show pulmonary and renal inflammation. ? Novel SS autoantibodies are elevated in sera from NOD.B10 mice.