Streamlining chemical protein synthesis into one pot

摘要

Researchers at the University of Sydney have recently reported the implementation of 2,2,2-trifluoroethanethiol (TFET) as a highly efficient thiol additive for Use in one-pot native chemical ligation-desulfurisation chemistry (Thompson R.E., Liu X., Alonso-Garcia N., Pereira P.J.B., Jolliffe K.A., Payne R.J. J. Am. Chem. Soc. 2014, 136, 8161-4). Importantly, this new technology can be applied to the total chemical synthesis of proteins that do not possess cysteine residues at suitable sites for assembly through traditional ligation techniques.