Optical Detection of Photorelease Kinetics on Gold and Glass Surfaces using Streptavidin‐Coupled Biotinylated Photolabile Protecting Groups for Nucleosides

Drexler Katja; Smirnova Julia; Galetskaya Marina; Schweizer Nina; Gauglitz Günter.; Steiner Ulrich E.

摘要

Abstract > Five biotinylated photolabile compounds of the general structure Bt‐L <sub>1</sub> ‐NPPOC‐X‐L <sub>2</sub> were synthesized, in which Bt represents a biotin unit, L <sub>1</sub> is a 3,6‐dioxa‐ n ‐octane or an n ‐hexane spacer, NPPOC is the photolabile protecting group 2‐(2‐nitrophenyl)propoxycarbonyl, and X is a thymidine unit as a representative nucleoside or a direct linkage to L <sub>2</sub> , an ω‐mercapto‐ or ω‐aminohexoyl linker, for coupling to a substrate surface. These compounds served for testing the photocleavage kinetics in self‐assembled monolayers on gold or glass by using surface plasmon resonance (SPR) on gold or reflectometric interference spectroscopy (RIfS) on glass, whereby the biotin moiety offered the possibility to increase the bulkiness of the leaving group by binding to streptavidin, which thereby largely enhanced the SPR or RIfS signals. The photokinetics, found to consist in a dominating fast stage and a less contributing slow stage, were quantitatively analyzed, and the quantum yield of the fast part reached values up to almost 1 in favorable cases. A direct comparison of the results from SPR and RIfS yielded almost identical results. The present investigations pave the way to in?situ monitoring of the photolithographic synthesis of DNA chips. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>摘要</标题> >一般结构的五种生物素化的光电子化合物BT-L <SUB> 1 </ sub>合成-NPPoc-X-L <亚> 2 </ sup>，其中BT代表生物素单元，L <sub> 1 </ sub>是3,6-二氧化硅 - n </ i> - 辛烷或 -hexane间隔物，NPPoc是光光保护基团2-（2-硝基苯基）丙氧基羰基，X是胸苷单元作为代表性核苷或直接连接到L <sub> 2 </ sub>，ω-巯基或ω-氨基氧酰基连接器，用于耦合到基板表面。这些化合物用于通过在玻璃上使用表面等离子体共振（SPR）在金色或玻璃上的自组装单层上的光敏动力学在金色或玻璃上进行测试，从而使生物素部分提供增加巨大的可能性通过与链霉抗生物素蛋白结合，从而大大增强了SPR或RIFS信号。发现在主导的快速阶段和较少有助于慢阶段的光动力学被定量地分析，并且快速部分的量子产量在有利的情况下达到了几乎几乎1的值。从SPR和RIF的结果直接比较几乎相同的结果。本研究铺平了进入的方法，对DNA芯片的光刻合成的原位监测。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-16300/'>《Chemphyschem: A European journal of chemical physics and physical chemistry》</a> <b style="margin: 0 2px;">|</b><span>2017年第20期</span><b style="margin: 0 2px;">|</b><span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Drexler Katja&option=202" target="_blank" rel="nofollow">Drexler Katja;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Smirnova Julia&option=202" target="_blank" rel="nofollow">Smirnova Julia;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Galetskaya Marina&option=202" target="_blank" rel="nofollow">Galetskaya Marina;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Schweizer Nina&option=202" target="_blank" rel="nofollow">Schweizer Nina;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gauglitz Günter.&option=202" target="_blank" rel="nofollow">Gauglitz Günter.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Steiner Ulrich E.&option=202" target="_blank" rel="nofollow">Steiner Ulrich E.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Fachbereich ChemieUniversit?t KonstanzUniversit?tsstra?e 10 78457 Konstanz Germany) Fax: (+49)?7531-88-3014;</p> <p>Fachbereich ChemieUniversit?t KonstanzUniversit?tsstra?e 10 78457 Konstanz Germany) Fax: (+49)?7531-88-3014;</p> <p>Fachbereich ChemieUniversit?t KonstanzUniversit?tsstra?e 10 78457 Konstanz Germany) Fax: (+49)?7531-88-3014;</p> <p>Institut für Physikalische und Theoretische ChemieUniversit?t TübingenAuf der Morgenstelle 18 72076 Tübingen Germany;</p> <p>Institut für Physikalische und Theoretische ChemieUniversit?t TübingenAuf der Morgenstelle 18 72076 Tübingen Germany;</p> <p>Fachbereich ChemieUniversit?t KonstanzUniversit?tsstra?e 10 78457 Konstanz Germany) Fax: (+49)?7531-88-3014;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1186.html" title="物理化学（理论化学）、化学物理学">物理化学（理论化学）、化学物理学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=nucleosides&option=203" rel="nofollow">nucleosides;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=photochemistry&option=203" rel="nofollow">photochemistry;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=self-assembly&option=203" rel="nofollow">self-assembly;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=surface plasmon resonance&option=203" rel="nofollow">surface plasmon resonance;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=synthetic methods&option=203" rel="nofollow">synthetic methods;</a> </p> <div class="translation"> 机译：核苷;光化学;自组装;表面等离子体共振;合成方法; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> 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