Self Assembly through Sonication: An Expeditious and Green Approach for the Synthesis of Organic-Inorganic Hybrid Nanopetals and their Application as Biocatalyst

摘要

The present study exhibits a facile method for the expeditious synthesis of organic-inorganic hybrid nanoassemblies. Pseudomonas fluorescens lipase was immobilized on the nanoassembly and its application as a nanobiocatalyst was demonstrated. Scanning electron microscopy analysis depicted petal-like morphology of the newly synthesized hybrid. The sonochemical method screened as a faster route for the synthesis of hybrid nanopetals with a marked increase in enzyme activity (15 folds). Furthermore, an additional treatment of the hybrid nanopetals with chemical modifiers likes 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) exhibited improved stability and reusability upto six cycles. A systematic investigation of the reaction parameters influencing the formation of lipase-embedded hybrid nanopetals was done. In addition, the nanobiocatalytic potential of hybrid nanopetals was explored in the kinetic resolution of racemic secondary alcohols.