Development of silver nanoparticles-loaded CMC hydrogel using bamboo as a raw material for special medical applications

摘要

The present investigation focuses on the synthesis of silver nanoparticles (Ag-NPs)-loaded hydrogel from synthetic and lab-made carboxymethyl cellulose (CMC) using bamboo as a raw material. The Ag-NPs were made by in situ process by the reaction of CMC and epichlorohydrin in alkaline medium containing silver nitrate. CMC acts as a stabilizer and reducing agent in the formation of nanoparticles. The formation of Ag-NPs was confirmed by UV-Vis spectrophotometer, as an absorption peak around 425 nm was observed. The Ag-NPs antibacterial activity against Escherichia coli (ATCC 433), Bacillus subtilis (ATCC 1688) was calculated by agar disk diffusion technique method. The characterization of the Ag-NPs was done by FTIR, SEM, TEM, particle size analyzer and EDX. Ag-NPs obtained from lab-made CMC displayed a higher antibacterial action towards Gram-negative (Escherichia coli) bacteria as well as Gram-Positive bacteria (Bacillus subtilis) as compared to synthetic CMC obtained from market.